Molecular analysis of smooth muscle development in the mouse
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TLDR
The results of this study indicate that distinct cellular phenotypes are involved in smooth muscle myogenesis and suggest that organ‐specific mechanisms might exist for the initiation of smooth muscle development in vivo.Abstract:
Little is currently known regarding the ontogeny of smooth muscle tissues during normal mammalian development. The alpha-smooth muscle and gamma-smooth muscle isoactins have been shown to be excellent molecular markers of smooth muscle cell phenotype. This study characterizes both the temporal and spatial patterns of alpha-smooth muscle and gamma-smooth muscle isoactin expression in the developing mouse. In situ analysis was performed on serial sections of whole mouse embryos on embryonic day 9, 11, 13, 15, and 17 using alpha-smooth muscle and gamma-smooth muscle isoactin-specific riboprobes. Distinct temporal and spatial patterns of alpha-smooth muscle and gamma-smooth muscle isoactin gene expression were observed in the developing gastrointestinal tract, urogenital tract, respiratory tract, and vascular system. Independent expression of the alpha-smooth muscle isoactin was observed during the early stages of skeletal, cardiac, and smooth muscle myogenesis as well as in a novel subset of distinct organs including the postnatal component of the hindgut, allantois, and primitive placenta. The results of this study indicate that distinct cellular phenotypes are involved in smooth muscle myogenesis and suggest that organ-specific mechanisms might exist for the initiation of smooth muscle development in vivo. In addition, the pattern of independent alpha-smooth muscle isoactin expression observed in this study provides novel information regarding the early stages of hindgut and placental development, and suggests that a common functional phenotype may be associated with the early stages of skeletal, cardiac, and smooth muscle myogenesis.read more
Citations
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Journal ArticleDOI
Smooth muscle γ-actin promoter regulation by RhoA and serum response factor signaling
James A. Carson,Donald E. Culberson,Donald E. Culberson,Raymond W. Thompson,Rebecca A. Fillmore,Warren E. Zimmer +5 more
TL;DR: As the SMGA promoter appears to be a target of RhoA-mediated transcriptional regulation, the uncovering of these signaling mechanisms effecting SMGA promoters should provide a regulatory paradigm that can be examined during the regulation of other smooth muscle genes.
Journal ArticleDOI
Developmental expression of neurokinin A and functional neurokinin-2 receptors in lung.
Kathleen J. Haley,Mary E. Sunday,Rapin Osathanondh,Juan Du,Charie Vathanaprida,Vladimir V. Karpitsky,James E. Krause,Craig M. Lilly +7 more
TL;DR: Results indicate that endogenous tachykinins released by the developing lung act via NK(2) receptors to cause smooth muscle constriction and speculate that tachy Kinins could modulate lung development.
Journal ArticleDOI
Immunocytochemical detection of synaptophysin in enteric neurones during prenatal development in the rat stomach.
TL;DR: Results indicate that synaptophysin is expressed in growing neurits and neuronal cell bodies before these neurones have established synaptic connections, and is functionally important in neuronal development and maturation.
Journal ArticleDOI
Establishment and characterization of a conditionally immortalized smooth muscle/myometrial-like cell line.
Jin Qian,E. Michael Hendrix,William J. Larsen,Gerald W. Dorn,James L. Lessard,James L. Lessard +5 more
TL;DR: At the nonpermissive temperature for SV40 large T‐antigen, the both level of SMGA mRNA and the number of cells staining for this actin are significantly increased in the presence of progesterone, a process that is similar to the upregulation ofSMGA in the myometrium late in pregnancy.
Journal ArticleDOI
Morphogenetic lung defects of JSAP1-deficient embryos proceeds via the disruptions of the normal expressions of cytoskeletal and chaperone proteins.
Hye Yeong Ha,Jung Bin Kim,Ik Hyun Cho,Hyo Jin Joo,Kyoung Shim Kim,Kang Woo Lee,Hongjai Sunwoo,Joo Young Im,Ja-Kyeong Lee,Jang Hee Hong,Pyung Lim Han +10 more
TL;DR: The results suggest that JSAP1 is required for the normal expressions of cytoskeletal and chaperone proteins in the developing lung, and that impaired expressions of these proteins might cause morphogenetic defects observed in jsap1−/− lungs.
References
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The smooth muscle cell in culture
TL;DR: The current state of knowledge of both vascular and visceral smooth muscle in cell and tissue culture and the variety of preparations used for different experimental purposes are described in this article, where the authors refer to organ culture of smooth muscle tissues only periodically.
Journal ArticleDOI
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Sequential activation of alpha-actin genes during avian cardiogenesis: vascular smooth muscle alpha-actin gene transcripts mark the onset of cardiomyocyte differentiation.
D L Ruzicka,Robert J. Schwartz +1 more
TL;DR: The specific expression of the vascular smooth muscle alpha- actin gene marks the onset of differentiation of cardiac cells and represents the first demonstration of coexpression of both smooth muscle and striated alpha-actin genes within myogenic cells.
Journal ArticleDOI
Expression of smooth muscle-specific proteins in myoepithelium and stromal myofibroblasts of normal and malignant human breast tissue.
Daniel Lazard,Xavier Sastre,Maria G. Frid,Marina A. Glukhova,Jean Paul Thiery,Victor E. Koteliansky +5 more
TL;DR: Myoepithelial cells and stromal myofibroblasts are epithelial and mesenchymal cells, respectively, which coordinately express a set of smooth muscle markers while maintaining their specific original features.
Expression of smooth muscle-specific proteins in myoepithelium and stromal myofibroblasts of normal and malignant human breast tissue (smooth muscle differentiation/breast carcinoma)
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