Molecular analysis of smooth muscle development in the mouse
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TLDR
The results of this study indicate that distinct cellular phenotypes are involved in smooth muscle myogenesis and suggest that organ‐specific mechanisms might exist for the initiation of smooth muscle development in vivo.Abstract:
Little is currently known regarding the ontogeny of smooth muscle tissues during normal mammalian development. The alpha-smooth muscle and gamma-smooth muscle isoactins have been shown to be excellent molecular markers of smooth muscle cell phenotype. This study characterizes both the temporal and spatial patterns of alpha-smooth muscle and gamma-smooth muscle isoactin expression in the developing mouse. In situ analysis was performed on serial sections of whole mouse embryos on embryonic day 9, 11, 13, 15, and 17 using alpha-smooth muscle and gamma-smooth muscle isoactin-specific riboprobes. Distinct temporal and spatial patterns of alpha-smooth muscle and gamma-smooth muscle isoactin gene expression were observed in the developing gastrointestinal tract, urogenital tract, respiratory tract, and vascular system. Independent expression of the alpha-smooth muscle isoactin was observed during the early stages of skeletal, cardiac, and smooth muscle myogenesis as well as in a novel subset of distinct organs including the postnatal component of the hindgut, allantois, and primitive placenta. The results of this study indicate that distinct cellular phenotypes are involved in smooth muscle myogenesis and suggest that organ-specific mechanisms might exist for the initiation of smooth muscle development in vivo. In addition, the pattern of independent alpha-smooth muscle isoactin expression observed in this study provides novel information regarding the early stages of hindgut and placental development, and suggests that a common functional phenotype may be associated with the early stages of skeletal, cardiac, and smooth muscle myogenesis.read more
Citations
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Journal ArticleDOI
microRNA-133a regulates cardiomyocyte proliferation and suppresses smooth muscle gene expression in the heart
Ning Liu,Svetlana Bezprozvannaya,Andrew H. Williams,Xiaoxia Qi,James A. Richardson,Rhonda Bassel-Duby,Eric N. Olson +6 more
TL;DR: Findings point to these miRNAs as critical components of an SRF-dependent myogenic transcriptional circuit in orchestrating cardiac development, gene expression, and function.
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MicroRNA Regulatory Networks in Cardiovascular Development
Ning Liu,Eric N. Olson +1 more
TL;DR: The roles of miRNAs as regulators of cardiac form and function, unresolved questions in the field, and issues for the future are discussed.
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Nox1 overexpression potentiates angiotensin ii-induced hypertension and vascular smooth muscle hypertrophy in transgenic mice
Anna Dikalova,Roza E. Clempus,Bernard Lassègue,Guangjie Cheng,James McCoy,Sergey Dikalov,Alejandra San Martin,Alicia N. Lyle,David S. Weber,Daiana Weiss,W. Robert Taylor,Harald H.H.W. Schmidt,Gary K. Owens,J. David Lambeth,Kathy K. Griendling +14 more
TL;DR: Data indicate that smooth muscle-specific Nox1 overexpression augments the oxidative, pressor, and hypertrophic responses to Ang II, supporting the concept that medial Nox 1 participates in the development of cardiovascular pathologies.
Journal ArticleDOI
Sonic hedgehog regulates proliferation and differentiation of mesenchymal cells in the mouse metanephric kidney.
TL;DR: In vivo and in vitro analyses demonstrate that sonic hedgehog promotes mesenchymal cell proliferation, regulates the timing of differentiation of smooth muscle progenitor cells, and sets the pattern of mesenchyme differentiation through its dose-dependent inhibition of smoother muscle formation.
Expression of smooth muscle-specific proteins in myoepithelium and stromal myofibroblasts of normal and malignant human breast tissue (smooth muscle differentiation/breast carcinoma)
TL;DR: The expression of several differentiation markers in normal human mammary gland myoepithelium and in certain stromal fibroblasts associated with breast carcinomas was studied by immunofluorescence microscopy of frozen sections as discussed by the authors.
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