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Book ChapterDOI

Molecular breeding of flower color of Torenia hybrida

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TLDR
Flower color is predominantly influenced by two types of pigments; flavonoids and carotenoids and the presence of flavonoid 3′5′-hydroxylase, cytochrome P-450, is almost critical to the production of blue to purple anthocyanins.
Abstract
Molecular breeding is a powerful method of plant breeding because it can change a specific characteristic of a plant without changing other desirable characteristics. Flower color is predominantly influenced by two types of pigments; flavonoids and carotenoids. The anthocyanin biosynthetic pathways of many plants have been well established (Holton et al, 1995) and conserved (Fig. 1). Chalcone synthase (CHS) and dihydroflavonol 4-reductase (DFR) are the first specific enzymes in flavonoid and anthocyanin biosynthesis, respectively. The presence of flavonoid 3′5′-hydroxylase (F3′5′H), cytochrome P-450 (Holton et al, 1993), is almost critical to the production of blue to purple anthocyanins. The flower color is reddish in its absence.

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Journal ArticleDOI

The Balance of Expression of Dihydroflavonol 4-reductase and Flavonol Synthase Regulates Flavonoid Biosynthesis and Red Foliage Coloration in Crabapples

TL;DR: The results suggest that the relative activities of McDFR and McFLS are important determinants of the red color of crabapple leaves, via the regulation of the metabolic fate of substrates that these enzymes have in common.
Journal ArticleDOI

Functional analyses of a flavonol synthase-like gene from Camellia nitidissima reveal its roles in flavonoid metabolism during floral pigmentation

TL;DR: In this article, the secondary metabolites of pigments during the development of Camellia nitidissima were characterized and revealed that accumulation of flavonols correlates with floral coloration.
Journal ArticleDOI

Isolation of Dihydroflavonol-4-reductase (DFR) Gene in Dendrobium helix cv. Pomeo Brown

TL;DR: The DFR gene from D. Pomeo Brown is analyzed by PCR using primer which were designed based on Dendrobium moniliforme, and it can be suggested that the amplified DNA consists of several DFR fragments.
References
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Journal ArticleDOI

Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans.

TL;DR: Somatic reversion of plants with white flowers to phenotypically parental violet flowers was associated with a coordinate rise in the steady-state levels of the mRNAs produced by both the endogenous and the introduced CHS genes, indicating that expression of the introduced chalcone synthase gene was not alone sufficient for suppression of endogenous CHS transcript levels.
Journal ArticleDOI

Genetics and Biochemistry of Anthocyanin Biosynthesis

TL;DR: The characterization of genetically defined mutations has enabled the order of many reactions in anthocyanin synthesis and their modification to be elucidated, and the more recent developments in gene isolation and characterization are concentrated.

Genetics and Biochemistry of Ant hocyanin Biosynthesis

TL;DR: In this paper, the authors focus on the more recent developments in gene isolation and characterization of anthocyanin biosynthetic genes and study their interactions and regulation in different species of maize, snapdragon, and petunia.
Journal ArticleDOI

Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants.

TL;DR: In transgenic tobacco plants, a representative powerful promoter, as compared to the 35S promoter, allowed 10- and 50-fold higher levels of expression on average and at most, respectively, with no clear qualitative differences in tissue- and organ-specific patterns of expression.
Journal ArticleDOI

An anti-sense chalcone synthase gene in transgenic plants inhibits flower pigmentation

TL;DR: It is shown that constitutive expression of an 'anti-sense' chalcone synthase gene in transgenic petunia and tobacco plants results in an altered flower pigmentation due to a reduction in levels of both the messenger RNA for the enzyme and the enzyme itself.
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