Journal ArticleDOI
Molecular characterization of a novel mitovirus from the plant‑pathogenic fungus Botryosphaeria dothidea.
TLDR
Phylogenetic analysis suggested that BdMV1 is a new member of the genus Mitovirus within the family Mitoviridae, the first report of a mitovirus in B. dothidea.Abstract:
Here, a novel mycovirus, Botryosphaeria dothidea mitovirus 1 (BdMV1), was isolated from a phytopathogenic fungus, Botryosphaeria dothidea, and its molecular characteristics were determined. BdMV1 has a genome of 2,667 nt that contains a single large open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF encodes an RNA-dependent RNA polymerase (RdRp) of 727 amino acids with a molecular mass of 81.64 kDa. BLASTp analysis revealed that the RdRp domain of BdMV1 has 39.59% and 39.18% sequence identity to Plasmopara viticola associated mitovirus 43 and Setosphaeria turcica mitovirus 1, respectively. Phylogenetic analysis further suggested that BdMV1 is a new member of the genus Mitovirus within the family Mitoviridae. To the best of our knowledge, this is the first report of a mitovirus in B. dothidea.read more
Citations
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Journal ArticleDOI
Characterization of the Mycovirome of the Phytopathogenic Fungus, Neofusicoccum parvum.
Armelle Marais,Chantal Faure,Gwenaëlle Comont,Thierry Candresse,Elodie Stempien,Marie-France Corio-Costet +5 more
TL;DR: In this paper, the mycovirome of a single strain of N. parvum (COLB) was characterized by high throughput sequencing analysis of total RNA and subsequent bioinformatic analyses.
Journal ArticleDOI
Molecular characterization of a new fusarivirus infecting Botryosphaeria dothidea, the causal agent of pear ring rot disease
TL;DR: Sequence alignments and phylogenetic analysis indicated that BdFV2 is a distinct member of the recently established family Fusariviridae, and appears to be a novel fUSarivirus infecting a pathogenic B. dothidea strain that causes pear ring rot disease.
Journal ArticleDOI
Characterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants
Journal ArticleDOI
Six Novel Mycoviruses Containing Positive Single-Stranded RNA and Double-Stranded RNA Genomes Co-Infect a Single Strain of the Rhizoctonia solani AG-3 PT
TL;DR: It is confirmed that RsPV10 andRsPV11 assemble into the spherical virus particles that were extracted from strain ZJ-2H, the first report that +ssRNA and dsRNA viruses co-infect a single strain of R. solani AG-3 PT.
Journal ArticleDOI
Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea
TL;DR: A novel double-stranded RNA (dsRNA) mycovirus, tentatively named Botryosphaeria dothidea partitivirus 2 (BdPV2), was identified well and indicated that it belonged to the Gammapartitiv virus genus and Partitiviridae family, the first report of a Gamsapart itivirus in B. dothsidea.
References
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Journal ArticleDOI
MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets
TL;DR: The latest version of the Molecular Evolutionary Genetics Analysis (Mega) software, which contains many sophisticated methods and tools for phylogenomics and phylomedicine, has been optimized for use on 64-bit computing systems for analyzing larger datasets.
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