Molecular characterization of Campylobacter jejuni clones: a basis for epidemiologic investigation.
Kate E. Dingle,Frances M. Colles,Roisin Ure,Jaap A. Wagenaar,Birgitta Duim,F. J. Bolton,Andrew J. Fox,David R. A. Wareing,Martin C. J. Maiden +8 more
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TLDR
The data demonstrate that the clonal complex, as defined by MLST, is an epidemiologically relevant unit for both long and short-term investigations of C. jejuni epidemiology, and several clonal complexes exhibited associations with isolation source or particular cell-surface components.Abstract:
A total of 814 isolates of the foodborne pathogen Campylobacter jejuni were characterized by multilocus sequence typing (MLST) and analysis of the variation of two cell-surface components: the heat-stable (HS) serotyping antigen and the flagella protein FlaA short variable region (SVR). We identified 379 combinations of the MLST loci (sequence types) and 215 combinations of the cell-surface components among these isolates, which had been obtained from human disease, animals, food, and the environment. Despite this diversity, 748 (92%) of the isolates belonged to one of 17 clonal complexes, 6 of which contained many (318, 63%) of the human disease isolates. Several clonal complexes exhibited associations with isolation source or particular cell-surface components; however, the latter were poorly predictive of clonal complex. These data demonstrate that the clonal complex, as defined by MLST, is an epidemiologically relevant unit for both long and short-term investigations of C. jejuni epidemiology.read more
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Multilocus Sequence Typing of Bacteria
TL;DR: Multilocus sequence typing (MLST) was proposed in 1998 as a portable, universal, and definitive method for characterizing bacteria, using the human pathogen Neisseria meningitidis as an example.
Journal ArticleDOI
Campylobacters as zoonotic pathogens: A food production perspective
TL;DR: Campylobacter (C.) jejuni and C. coli are the two species which are most important in food-borne infections of humans, which infect an estimated 1% of the population of Western Europe each year as mentioned in this paper.
Journal ArticleDOI
Multi-locus sequence typing: a tool for global epidemiology
Rachel Urwin,Martin C. J. Maiden +1 more
TL;DR: Multi-locus sequence typing was proposed as a nucleotide sequence-based approach that could be applied to many bacterial pathogens to provide a portable, reproducible, and scalable typing system that reflected the population and evolutionary biology of bacterial pathogens.
Journal ArticleDOI
Major Structural Differences and Novel Potential Virulence Mechanisms from the Genomes of Multiple Campylobacter Species
Derrick E. Fouts,Emmanuel F. Mongodin,Robert E. Mandrell,William G. Miller,David A. Rasko,Jacques Ravel,Lauren M. Brinkac,Robert T. DeBoy,Craig T. Parker,Sean C. Daugherty,Robert J. Dodson,A. Scott Durkin,Ramana Madupu,Steven A. Sullivan,Jyoti Shetty,Mobolanle A Ayodeji,Alla Shvartsbeyn,Michael C. Schatz,Jonathan H. Badger,Claire M. Fraser,Karen E. Nelson +20 more
TL;DR: Sequencing and comparative genome analysis of four strains of Campylobacter has revealed major structural differences that are associated with the insertion of phage- and plasmid-like genomic islands, as well as major variations in the lipooligosaccharide complex.
Journal ArticleDOI
Tracing the source of campylobacteriosis.
Daniel J. Wilson,Edith Gabriel,A. J. H. Leatherbarrow,John Cheesbrough,Steven Gee,Eric Bolton,Andrew D. Fox,Andrew D. Fox,Paul Fearnhead,C. Anthony Hart,Peter J. Diggle +10 more
TL;DR: The novel population genetics approach reveals that the vast majority (97%) of sporadic disease can be attributed to animals farmed for meat and poultry, whereas wild animal and environmental sources are responsible for just 3% of disease.
References
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Journal ArticleDOI
Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganisms
Martin C. J. Maiden,Jane A. Bygraves,Edward J. Feil,Giovanna Morelli,Joanne E. Russell,Rachel Urwin,Qing Zhang,Jiaji Zhou,Kerstin Zurth,Dominique A. Caugant,Ian M. Feavers,Mark Achtman,Brian G. Spratt +12 more
TL;DR: Multilocus sequence typing (MLST), which exploits the unambiguous nature and electronic portability of nucleotide sequence data for the characterization of microorganisms, can be applied to almost all bacterial species and other haploid organisms, including those that are difficult to cultivate.
Journal ArticleDOI
Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus.
Mark C. Enright,Nicholas P. J. Day,Catrin E. Davies,Sharon J Peacock,Sharon J Peacock,Brian G. Spratt +5 more
TL;DR: A multilocus sequence typing (MLST) scheme has been developed for Staphylococcus aureus and provides an unambiguous method for assigning MRSA and MSSA isolates to known clones or assigning them as novel clones via the Internet.
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SplitsTree: analyzing and visualizing evolutionary data.
TL;DR: SplitsTree is an interactive program, for analyzing and visualizing evolutionary data, that implements the method of split decomposition, and supports a number of distances transformations, the computation of parsimony splits, spectral analysis and bootstrapping.
Journal ArticleDOI
A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease
Mark C. Enright,Brian G. Spratt +1 more
TL;DR: A pneumococcal multilocus sequence typing scheme and database is developed by sequencing approximately 450 bp fragments of seven housekeeping loci from 295 isolates to provide an allelic profile, or sequence type (ST), and the relatedness between isolates was obtained by constructing a dendrogram from the matrix of pairwise differences between STs.