Molecular cloning and mapping of phenol degradation genes from Bacillus stearothermophilus FDTP-3 and their expression in Escherichia coli.
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TLDR
Two genes of the meta pathway of phenol degradation were cloned from a phenol-utilizing strain of Bacillus stearothermophilus and were mapped by subcloning and by use of a Tn5 insertion mutation.Abstract:
Two genes of the meta pathway of phenol degradation were cloned from a phenol-utilizing strain of Bacillus stearothermophilus and were mapped by subcloning and by use of a Tn5 insertion mutation. They code for phenol hydroxylase and catechol 2,3-dioxygenase, respectively. The gene encoding catechol 2,3-dioxygenase, which is more thermostable than catechol 2,3-dioxygenase encoded by the other gene, shares rather limited homology with that from Pseudomonas putida.read more
Citations
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2,4-Dichlorophenoxyacetic acid-degrading bacteria contain mosaics of catabolic genes.
TL;DR: The data suggest that extensive interspecies transfer of a variety of homologous degradative genes has been involved in the evolution of 2,4-D-degrading bacteria.
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A manganese-dependent dioxygenase from Arthrobacter globiformis CM-2 belongs to the major extradiol dioxygenase family.
TL;DR: The first available sequence for a manganese-dependent 3, 4-dihydroxyphenylacetate (3,4-DHPA) 2,3-dioxygenase and its further characterization is reported and it is suggested that MndD belongs to the extradiol family of dIOxygenases and may share a common ancestry with the iron-dependent extradiol dioXYgenases.
Journal ArticleDOI
Design of PCR primers and gene probes for the general detection of bacterial populations capable of degrading aromatic compounds via catechol cleavage pathways
TL;DR: The results suggest that the primer and probe systems can detect a considerable proportion of bacteria which can degrade aromatic compounds via catechol cleavage pathways newly isolated from a variety of environments.
Journal ArticleDOI
Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis
Le Thi Tam,Christine Eymann,Dirk Albrecht,Rabea Sietmann,Frieder Schauer,Michael Hecker,Haike Antelmann +6 more
TL;DR: The studies revealed that the catechol-2,3-dioxygenase YfiE is the key enzyme of a meta cleavage pathway in B. subtilis involved in the catabolism of catechl.
Journal ArticleDOI
PCR isolation of catechol 2,3-dioxygenase gene fragments from environmental samples and their assembly into functional genes
TL;DR: A method was developed to isolate central segments of catechol 2, 3-dioxygenase (C23O) genes from environmental samples and to insert these C23O gene segments into nahH by replacing the corresponding nah H sequence with the isolated segments.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
Journal ArticleDOI
Preparation of transforming deoxyribonucleic acid by phenol treatment
Hiuga Saito,Kin-ichiro Miura +1 more
TL;DR: The final DNA preparation has high transforming activity, especially for the joint transformation of linked markers, as well as high purity and low sedimentation coefficients.
Journal ArticleDOI
A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.
TL;DR: A cleavage site map of Tn5 for restriction enzymes BamHI, B glI, BglII, Hind II, HindIII, HpaI, SalI, AvaI, SmAI, XhoI, PstI, PvuII, HaeII and HaeIII that was determined by the analysis of restriction enzyme cleavage patterns of ColEl.
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