Journal ArticleDOI
Molecular typing of Helicobacter pylori isolates from a multicenter U.S. clinical trial by ureC restriction fragment length polymorphism.
Virginia D. Shortridge,Gregory G. Stone,Robert K. Flamm,J. Beyer,James Versalovic,D. W. Graham,S. K. Tanaka +6 more
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TLDR
The results of this study show the extreme genomic diversity of H. pylori and indicate that infection by H.pylori in the United States does not appear to be limited to a small number of RFLP types.Abstract:
The molecular typing of 81 pretreatment Helicobacter pylori isolates and the comparison of 18 pretreatment-posttreatment pairs is described by restriction fragment length polymorphism (RFLP) of the ureC gene. The results of our study show the extreme genomic diversity of H. pylori and indicate that infection by H. pylori in the United States does not appear to be limited to a small number of RFLP types.read more
Citations
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Principles and Applications of Methods for DNA-Based Typing of Microbial Organisms
D. Michael Olive,Pamela Bean +1 more
TL;DR: Outbreaks of infectious disease often result from exposure to a common source of the etiologic agent, which is derived from a single cell whose progeny are genetically identical or closely related to the source organism.
Journal ArticleDOI
A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates.
TL;DR: A toxinotype was characterized by similar patterns of changes in the toxin genes and in other regions of the pathogenicity locus and also similar pulsed-field gel electrophoresis patterns.
Journal ArticleDOI
Bacterial population genetics, evolution and epidemiology
TL;DR: The analysis of collections ofacterial isolates by nucleotide sequence determination of multiple housekeeping loci provides data that can be used both to investigate the population structure of bacterial pathogens and for the molecular characterization of bacterial isolates.
Journal ArticleDOI
Analysis of the genetic diversity of Helicobacter pylori: the tale of two genomes.
Richard A. Alm,Trevor J. Trust +1 more
TL;DR: Analysis of two completed genome sequences from unrelated strains indicates that the H. pylori specific genes have a lower level of conservation than those orthologues to which a putative function can be assigned, and implies a need to reassess the respective roles of bacterial and host factors in H.pylori associated diseases.
Journal ArticleDOI
Antibiotic Resistance of Helicobacter pylori Strains in Japanese Children
Seiichi Kato,Shigeru Fujimura,Hirokazu Udagawa,Toshiaki Shimizu,Shunichi Maisawa,Kyoko Ozawa,Kazuie Iinuma +6 more
TL;DR: The results indicate that a high prevalence of clarithromycin-resistant strains is associated with eradication failure, and testing of susceptibility to clarityromycin is recommended.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal Article
Infection with Helicobacter pylori Strains Possessing cagA Is Associated with an Increased Risk of Developing Adenocarcinoma of the Stomach
Martin J. Blaser,Guillermo I. Perez-Perez,Harry Kleanthous,Timothy L. Cover,Richard M. Peek,Po-Huang Chyou,Grant N. Stemmermann,Abraham M. Y. Nomura +7 more
TL;DR: It is concluded that infection with a cagA-positive H. pylori strain in comparison with acagA -negative strain somewhat increases the risk for development of gastric cancer, especially intestinal type affecting the distal stomach.
Journal ArticleDOI
Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production.
TL;DR: Findings indicate the cloning and characterization of a high-molecular-mass H. pylori antigen potentially associated with virulence and with cytotoxin production.
Journal ArticleDOI
Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains.
TL;DR: Sequencing data suggest that sequence divergence in vacA genes may explain the lack of functionally active cytotoxin production by some H. pylori isolates.
Journal ArticleDOI
Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori.
James Versalovic,D. Shortridge,K. Kibler,M. V. Griffy,J. Beyer,R. K. Flamm,S. K. Tanaka,David Y. Graham,Mae F. Go +8 more
TL;DR: This study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens and shows no polymorphisms in a conserved loop within domain V of 23S rRNA.