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Journal ArticleDOI

msatcommander: detection of microsatellite repeat arrays and automated, locus-specific primer design

Brant C. Faircloth
- 01 Jan 2008 - 
- Vol. 8, Iss: 1, pp 92-94
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TLDR
msatcommander is a platform‐independent program designed to search for microsatellite arrays, design primers, and tag primers using an automated routine, using single‐sequence or concatenated, fasta‐formatted files.
Abstract
msatcommander is a platform-independent program designed to search for microsatellite arrays, design primers, and tag primers using an automated routine. msatcommander accepts as input DNA sequence data in single-sequence or concatenated, fasta-formatted files. Search data and locus-specific primers are written to comma-separated value files for subsequent use in spreadsheet or database programs. Binary versions of the graphical interface for msatcommander are available for Apple OS X and Windows XP. Users of other operating systems may run the graphical interface version using the available source code, provided their environment supports at least Python 2.4, Biopython 1.43, and wxPython 2.8. msatcommander is available from http://code.google.com/p/msatcommander/.

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Citations
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Journal ArticleDOI

Primer3—new capabilities and interfaces

TL;DR: Primer3’s current capabilities are described, including more accurate thermodynamic models in the primer design process, both to improve melting temperature prediction and to reduce the likelihood that primers will form hairpins or dimers.
Journal ArticleDOI

Applications of next generation sequencing in molecular ecology of non-model organisms

TL;DR: A snapshot of the current state of the art for this rapidly advancing and expanding field of research is provided and some likely directions for future developments are given.
Journal ArticleDOI

Current trends in microsatellite genotyping.

TL;DR: To make the most of the latest technical developments, the need for a well‐established strategy including standardized high‐throughput bench protocols and specific bioinformatic tools, from primer design to allele calling is outlined.
Journal ArticleDOI

Fast, cost-effective development of species-specific microsatellite markers by genomic sequencing

TL;DR: This study uses high-throughput genomic sequencing technology to produce millions of base pairs of short fragment reads, which were screened with bioinformatics toolsets to identify primers that amplify polymorphic microsatellite loci.
Journal ArticleDOI

Rise of the machines--recommendations for ecologists when using next generation sequencing for microsatellite development.

TL;DR: There are clear differences amongst plants, invertebrates and vertebrates for the number and proportion of motif types recovered that are able to be utilized as markers and it is highlighted that the heterogeneity within each group is very large.
References
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Book ChapterDOI

Primer3 on the WWW for general users and for biologist programmers.

TL;DR: This chapter assumes acquaintance with the principles and practice of PCR, as outlined in, for example, refs.
Book ChapterDOI

Isolating microsatellite DNA loci

TL;DR: A series of techniques are presented to construct genomic DNA libraries highly enriched for microsatellite DNA loci, making it possible to reduce the time to identify candidate loci for primer development from most eukaryotic species to as little as 1 week.
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