Journal ArticleDOI
New insights into the regulation and cellular functions of the ARP2/3 complex
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TLDR
The mechanisms underlying NPF-dependent regulation and the cellular functions of ARP2/3 are being unravelled using new chemical and genetic approaches, of particular interest is the role of the ARP/3 complex in vesicular trafficking and directional cell motility.Abstract:
The actin-related protein 2/3 (ARP2/3) complex nucleates branched actin filament networks, but requires nucleation promoting factors (NPFs) to stimulate this activity. NPFs include proteins such as Wiskott-Aldrich syndrome protein (WASP), neural WASP (NWASP), WASP family verprolin-homologous protein (WAVE; also known as SCAR) and the recently identified WASP and SCAR homologue (WASH) complex. The mechanisms underlying NPF-dependent regulation and the cellular functions of ARP2/3 are being unravelled using new chemical and genetic approaches. Of particular interest is the role of the ARP2/3 complex in vesicular trafficking and directional cell motility.read more
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Dissertation
Synthetic Lethal Interactions with E-cadherin for Cancer Therapy
TL;DR: A whole genome siRNA screen and a high throughput known drug screen are described to identify genes and drugs which cause a greater reduction in viability inCDH1 cells, compared to the CDH1 wild-type.
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Antiviral Response by Plasmacytoid Dendritic Cells via Interferogenic Synapse with Infected Cells
Sonia Assil,Séverin Coléon,Elodie Décembre,Lee Sherry,Omran Allatif,Brian Webster,Marlène Dreux +6 more
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Arp2/3 complex activity is necessary for mouse ESC differentiation, times formative pluripotency, and enables lineage specification
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Original Article Investigating the role of the actin regulating complex ARP2/3 in rapid ischemic tolerance induced neuro-protection
Veronica J Jessick,Mian Xie,Andrea N Pearson,Dan J Torrey,Michelle D Ashley,Simon Thompson,Robert Meller,S. Dow +7 more
TL;DR: In this article, the role of actin regulating proteins (ARP2, ARP3 and WAVE-1) and their role in rapid ischemic tolerance was investigated.
Posted ContentDOI
Adherens junction serves to generate cryptic lamellipodia required for collective migration of epithelial cells
Masayuki Ozawa,Sylvain Hiver,Takaki Yamamoto,Tatsuo Shibata,Srigokul Upadhyayula,Yuko Mimori-Kiyosue,Masatoshi Takeichi +6 more
TL;DR: It is concluded that AJs not only tie cells together but also generate c-lamellipodia by recruiting actin regulators, enabling epithelial cells to undergo ordered collective migration.
References
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Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Regulation of Actin Filament Assembly by Arp2/3 Complex and Formins
TL;DR: This review summarizes what is known about the biochemical and biophysical mechanisms that initiate the assembly of actin filaments in cells and focuses on Arp2/3 complex and formins.
Journal ArticleDOI
The ARP2/3 complex: an actin nucleator comes of age
Erin D. Goley,Matthew D. Welch +1 more
TL;DR: A decade of study has begun to shed light on the molecular mechanisms by which this powerful machine controls the polymerization, organization and recycling of actin-filament networks, both in vitro and in the living cell.
Journal ArticleDOI
A nucleator arms race: cellular control of actin assembly
TL;DR: The Spire, cordon-bleu and leiomodin nucleators have revealed new ways of overcoming the kinetic barriers to actin polymerization.
Journal ArticleDOI
Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck
TL;DR: It is proposed that Rac1 and Nck cause dissociation of the WAVE1 complex, which releases activeWAVE1–HSPC300 and leads to actin nucleation.