Journal ArticleDOI
New insights into the regulation and cellular functions of the ARP2/3 complex
Reads0
Chats0
TLDR
The mechanisms underlying NPF-dependent regulation and the cellular functions of ARP2/3 are being unravelled using new chemical and genetic approaches, of particular interest is the role of the ARP/3 complex in vesicular trafficking and directional cell motility.Abstract:
The actin-related protein 2/3 (ARP2/3) complex nucleates branched actin filament networks, but requires nucleation promoting factors (NPFs) to stimulate this activity. NPFs include proteins such as Wiskott-Aldrich syndrome protein (WASP), neural WASP (NWASP), WASP family verprolin-homologous protein (WAVE; also known as SCAR) and the recently identified WASP and SCAR homologue (WASH) complex. The mechanisms underlying NPF-dependent regulation and the cellular functions of ARP2/3 are being unravelled using new chemical and genetic approaches. Of particular interest is the role of the ARP2/3 complex in vesicular trafficking and directional cell motility.read more
Citations
More filters
Journal ArticleDOI
Sensitized mutagenesis screen in Factor V Leiden mice identifies thrombosis suppressor loci
Randal J. Westrick,Randal J. Westrick,Kärt Tomberg,Amy E. Siebert,Guojing Zhu,Mary E. Winn,Sarah L. Dobies,Sara L. Manning,Marisa A Brake,Audrey C. A. Cleuren,Linzi M Hobbls,Lena M Mishack,Alexander J Johnston,Emilee N. Kotnik,David R. Siemieniak,Jishu Xu,Jun Li,Thomas L. Saunders,David Ginsburg +18 more
TL;DR: F8 and the Tfpi/F3 axis as key regulators in determining thrombosis balance in the setting of F5L and also suggest a role for Actr2 in this process are identified, supporting a hypomorphic or gain-of-function mechanism of action for ActR2p.R258G.
Journal ArticleDOI
Plasma membrane localization of MLC1 regulates cellular morphology and motility.
TL;DR: The results suggest that misallocation of pathogenic mutant MLC1 may disturbs the stable cell-cell communication and the homeostatic regulation of astrocytes in patients with MLC.
Journal ArticleDOI
Hematopoietic cell-specific lyn substrate (HCLS1 or HS1): A versatile actin-binding protein in leukocytes.
TL;DR: Current knowledge on HS1 functions is summarized to discuss them in a pathophysiologic context and show why HS1 is important for the activation of GTPases and integrins, and mediates signaling downstream of many receptors including BCR, TCR, and CXCR4.
Journal ArticleDOI
Self-powered smart patch promotes skin nerve regeneration and sensation restoration by delivering biological-electrical signals in program.
TL;DR: Li et al. as mentioned in this paper proposed a self-powered smart patch (PRG-G-C) to provide chemokine and biological-electrical cues in program, which was composed of a flexible piezoelectric generator to supply electrical stimulation and a conductive gel, which served as the reservoir of chemokines and neural directing exosomes as well as the electrode to transfer electric cue.
Journal ArticleDOI
Molecular Dissection of Neurodevelopmental Disorder-Causing Mutations in CYFIP2.
TL;DR: The data show that explored CYFIP2 mutations frequently, but not always, coincide with WRC activation and suggest that normal brain development requires a delicate and precisely tuned balance of neuronal WRC activity.
References
More filters
Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Regulation of Actin Filament Assembly by Arp2/3 Complex and Formins
TL;DR: This review summarizes what is known about the biochemical and biophysical mechanisms that initiate the assembly of actin filaments in cells and focuses on Arp2/3 complex and formins.
Journal ArticleDOI
The ARP2/3 complex: an actin nucleator comes of age
Erin D. Goley,Matthew D. Welch +1 more
TL;DR: A decade of study has begun to shed light on the molecular mechanisms by which this powerful machine controls the polymerization, organization and recycling of actin-filament networks, both in vitro and in the living cell.
Journal ArticleDOI
A nucleator arms race: cellular control of actin assembly
TL;DR: The Spire, cordon-bleu and leiomodin nucleators have revealed new ways of overcoming the kinetic barriers to actin polymerization.
Journal ArticleDOI
Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck
TL;DR: It is proposed that Rac1 and Nck cause dissociation of the WAVE1 complex, which releases activeWAVE1–HSPC300 and leads to actin nucleation.