Open AccessJournal Article
New primer strategy improves precision of differential display
TLDR
Modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods to increase the reproducibility and sensitivity of the mRNA differentialdisplay while still keeping the characteristics of the original method.Abstract:
To increase the reproducibility and to reduce the false positives in the initial mRNA differential display, modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods. Ten-base nucleotides were added at the 5' ends of the primers used in the initial mRNA differential display. These included a restriction site to aid cloning. PCR began with one low-stringency cycle (40 degrees C for annealing) followed by 35 high-stringency cycles (60 degrees C for annealing). The modified method significantly improved the reproducibility and sensitivity of the mRNA differential display while still keeping the characteristics of the original method.read more
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Book ChapterDOI
Emerging Viruses: Their Diseases and Identification
TL;DR: The primary aim of this book is to identify any new infectious agent and build a body of data to support the existence of a causal link between organism and disease.
Book ChapterDOI
RNA differential display
TL;DR: Differential display is a rapid, easy-to-perform technique for the identification of genes whose mRNA accumulate to different levels in two or more tissue types and has been successful in identifying a large number of differentially accumulated transcripts.
Dissertation
Identification of genes encoding secreted proteins of schistosomes
TL;DR: In mice given a single vaccination with optimally irradiated S. mansoni cercariae, cellmediated mechanisms operating against lung-stage larvae are highly effective in reducing challenge worm burden by 60-70%, relative to those in control mice, suggesting a potential source of novel candidates for a recombinant vaccine.
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