Open AccessJournal Article
New primer strategy improves precision of differential display
TLDR
Modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods to increase the reproducibility and sensitivity of the mRNA differentialdisplay while still keeping the characteristics of the original method.Abstract:
To increase the reproducibility and to reduce the false positives in the initial mRNA differential display, modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods. Ten-base nucleotides were added at the 5' ends of the primers used in the initial mRNA differential display. These included a restriction site to aid cloning. PCR began with one low-stringency cycle (40 degrees C for annealing) followed by 35 high-stringency cycles (60 degrees C for annealing). The modified method significantly improved the reproducibility and sensitivity of the mRNA differential display while still keeping the characteristics of the original method.read more
Citations
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Dissertation
Aspects of gene expression and regulation in plasmodium falciparum gametocytogenesis
TL;DR: In this article, differential display was used to identify some of the genes that are differentially expressed between the asexual parasite and gametocyte stages of P. falciparum.
Journal ArticleDOI
Modified differential display technique to generate long cDNA fragments within the coding region.
Desarrollo de un protocolo de análisis de la expresión génica mediante «differential display» que reduce el número de falsos positivos
TL;DR: Modificaciones al protocolo nos ha permitido identificar 5 genes expresados diferencialmente entre celulas epiteliales de intestino en estado proliferativo y difererciado.
DDRT-PCR approaches applied for preeminent results in the isolation of DETs from fish brain tissues
TL;DR: This study indicates that some modifications on the DDRT-PCR method can ensure isolation of DETs from different fish tissues and the development of robust data related to this approach.
Patent
Transcription inverse, amplification, et leurs amorces
TL;DR: In this paper, a procede permettant -'etablir le profil d'expression de cellules isolees' was proposed, which concerne un procede which is based on a procedure that utilise a premiere amorce a " talon" (heeled) for the transcription inverse of ARNm dans un echantillon afin de produire des especes d'ADNc a premier brin, and puis une deuxieme population d'amorces a '' talon '' for generer
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