Open AccessJournal Article
New primer strategy improves precision of differential display
TLDR
Modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods to increase the reproducibility and sensitivity of the mRNA differentialdisplay while still keeping the characteristics of the original method.Abstract:
To increase the reproducibility and to reduce the false positives in the initial mRNA differential display, modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods. Ten-base nucleotides were added at the 5' ends of the primers used in the initial mRNA differential display. These included a restriction site to aid cloning. PCR began with one low-stringency cycle (40 degrees C for annealing) followed by 35 high-stringency cycles (60 degrees C for annealing). The modified method significantly improved the reproducibility and sensitivity of the mRNA differential display while still keeping the characteristics of the original method.read more
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Journal ArticleDOI
Differential Display of Genes in Normal and Hypoplastic Fetal Murine Lungs
TL;DR: Analysis of Northern blots of lungs from various gestational ages showed that the expression of NT5 was induced in hypoplastic lungs at Gd18, whereas in normal lungs it was first expressed at the neonatal stage and was increasingly expressed into adulthood.
Book ChapterDOI
Application of fluorescent differential display and peroxisome proliferator-activated receptor (PPAR)α-null mice to analyze PPAR target genes
TL;DR: This new DNA microarray method, in conjunction with the differential display method, is the first important step toward understanding the molecular mechanisms of gene interactions in any biological systems and can speed up the search for differential gene expressions.
Journal ArticleDOI
Changes in brain gene expression in psychiatric illness: mRNA differential display provides some clues
T P Flanigan,R A Leslie +1 more
TL;DR: The contribution of mRNA differential display to the analysis of altered gene expression in vitro and in vivo is considered and the role that this technique may play in the identification of genes involved in the aetiology of psychiatric disorders and their treatment is discussed.
Differential display: a novel pcr-based method for gene isolation and cloning 1
TL;DR: DDRT-PCR has the following essential steps: total RNA isolation and purification, cDNA synthesis from mRNAs, PCR amplification of cDNAs, visualization of PCR products, reamplification and cloning of the differentially expressed PCR products; and finally cDNA library screening to isolate the genes of interest.
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