Nucleotide sequence of cauliflower mosaic virus DNA
TLDR
The findings suggest, but do not prove, that the DNA sequence of the open reading frames is colinear with viral protein sequences, which is consistent with other evidence that only the alpha strand is transcribed.About:
This article is published in Cell.The article was published on 1980-08-01 and is currently open access. It has received 461 citations till now. The article focuses on the topics: Coding strand & Palindromic sequence.read more
Citations
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Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
TL;DR: The effects of 5′ deletions in a plant viral promoter in tobacco callus as well as in regenerated plants, includ ing different plant tissues, are analysed to allow a more direct assessment of deletion effects.
Journal ArticleDOI
Identification of four conserved motifs among the RNA-dependent polymerase encoding elements.
TL;DR: At the evolutionary level, the sequence similarities, gap distribution and distances between each motif strongly suggest that the ancestral polymerase module was encoded by an individual genetic element which was most closely related to the plus‐strand RNA viruses and the non‐viral retroposons.
Journal ArticleDOI
Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium.
TL;DR: It is shown here that aequorin can be reconstituted in transformed plants and that it reports calcium changes induced by touch, cold-shock and fungal elicitors, which could be valuable for determining the role of calcium in intracellular signalling processes in plants.
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Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes
TL;DR: A variant of the cauliflower mosaic virus 35S promoter with transcriptional activity approximately tenfold higher than that of the natural promoter was constructed by tandem duplication of 250 base pairs of upstream sequences, which should be very useful for obtaining high levels of expression of foreign genes in transgenic plants.
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Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae
Linda Gritz,Julian Davies +1 more
TL;DR: The plasmid-borne gene hph coding for hygromycin B phosphotransferase (HPH) in Escherichia coli has been identified and its nucleotide sequence determined.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
3′ Non-coding region sequences in eukaryotic messenger RNA
TL;DR: A large selection of the 3′ non-coding regions of rabbit and human globin mRN As are 85% homologous, demonstrating that this region is significantly conserved in evolution.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.
Journal ArticleDOI
Preparative and analytical purification of DNA from agarose.
Bert Vogelstein,David Gillespie +1 more
TL;DR: Two procedures were developed for removing DNA from agarose after electrophoretic separation of DNA fragments according to size, and the sequence-monitoring system is quantitative, directly measuring the proportion of the probe complementary to a given DNA fragment and vice versa.
Journal ArticleDOI
The use of thin acrylamide gels for DNA sequencing.
Frederick Sanger,Alan Coulson +1 more
TL;DR: Using the plus and minus [l] and the terminator [3] methods, serious variations in the distances between consecutive nucleotide bands in regions of dyad symmetry where base-paired loop structures can form are found, and this can lead to difficulties of interpretation.
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