Journal ArticleDOI
Oligosaccharide synthesis by reversed catalysis using α-amylase from Bacillus licheniformis
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In this paper, the authors used Termamyl, the thermotolerant α-amylase from Bacillus licheniformis, for the synthesis of hetero-oligosaccharides.Abstract:
Evidence is provided for the synthesis of hetero-oligosaccharides by Termamyl, the thermotolerant α-amylase from Bacillus licheniformis. In the presence of soluble starch and added non-starch sugars (isomaltose, cellobiose, mannose, melibiose, stachyose, xylose and 1- o -methyl-β- d -glucopyranoside), oligosaccharides were produced by the reversed catalytic reaction. New peaks of homo- and hetero-oligosaccharides (DP1-10) were detected by high performance anion exchange chromatography (HPAEC) in equilibrium reaction mixtures. Attempts to increase the extent of synthesis of oligosaccharides using organic solvents (ethanol, methanol, N - propanol, propanediol, N - butanol, dioxane and dimethyl sulfoxide (DMSO)) to lower the water activity of the reaction mixture were unsuccessful. Raising the temperature of the reaction mixture from 37°C to 55°C was ineffective.read more
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Developments in industrially important thermostable enzymes: a review.
TL;DR: The source microorganisms and properties of thermostable starch hydrolysing amylases, xylanases, cellulases, chitinases, proteases, lipases and DNA polymerases are discussed and the industrial needs for such specific thermostably enzyme and improvements required to maximize their application in the future are suggested.
Journal ArticleDOI
Action pattern and subsite mapping of Bacillus licheniformis α-amylase (BLA) with modified maltooligosaccharide substrates
TL;DR: Comparison of the binding energies of subsites shows that BLA has similarity to the closely related Bacillus amyloliquefaciens α‐amylase.
Journal ArticleDOI
Two Manganese Peroxidases and a Laccase of Trametes polyzona KU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System.
Piyangkun Lueangjaroenkit,Churapa Teerapatsakul,Kazuo Sakka,Makiko Sakka,Tetsuya Kimura,Emi Kunitake,Lerluck Chitradon +6 more
TL;DR: The efficacy of using T. polyzona KU-RNW027 for bioremediation of aromatic compounds in environments polluted with organic solvents and metal ions with no need for redox mediator supplements is indicated.
Journal ArticleDOI
Modification of stevioside using transglucosylation activity of Bacillus amyloliquefaciens α-amylase to reduce its bitter aftertaste
TL;DR: In this article, transglucosylation of stevioside by α-amylase from Bacillus amyloliquefaciens in the starch solution was investigated.
Patent
Grain fractionation methods and products
TL;DR: In this article, an organic solvent and water instead of water alone, acidified water and/or aqueous alkali as a solvent for the slurrying of a grain flour was used.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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TL;DR: A review about the potential of enzymes as catalysts for the synthesis of carbohydrates: advances in the enzyme-catalysed construction of natural and unnatural monosaccharides; methods for enzyme-Catalysed formation of glycosyl bonds by Lenoir and non-Lenoir pathways.
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Enzymatic synthesis of oligosaccharides
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A simple strategy for changing the regioselectivity of glycosidase-catalysed formation of disaccharides.
TL;DR: The regioselectivity of glycosidase-catalysed formation of disaccharides can be changed by using alpha- or beta-glycosyl acceptors with various aglycons, and an alpha-D-galactosidase can be induced to catalyse the formation mainly of alpha-(1----2)-, alpha-3, or alpha-1----6)-linked digalactosides.