Open AccessJournal Article
On the characteristics of actinomycin D resistance in L5178Y cells.
D Kessel,H B Bosmann +1 more
TLDR
A subline of the L5178Y murine leukemia was selected for resistance to actinomycin D by drug administration in vivo and in culture but not under nongrowing conditions, and altered cell surface glycoprotein fraction was found.Abstract:
Summary A subline of the L5178Y murine leukemia was selected for resistance to actinomycin D by drug administration in vivo . The resistant line, L5178Y/D, had impaired capacity for uptake of actinomycin D in vivo and in culture but not under nongrowing conditions. Administration of the drug in vivo (50 μg/kg) or in culture (0.1 μg/ml) inhibited uridine incorporation into RNA by L5178Y but not by L5178Y/D. An altered cell surface glycoprotein fraction was found in L5178Y/D. Enzymatic studies showed that levels of glycoprotein transferases catalyzing formation of amino acid-sugar and sugar-sugar bonds were generally higher, while specific activities of glycosidases were generally lower in L5178Y/D. Alterations in membrane composition and conformation in the drug-resistant subline could account for the observed changes in actinomycin D permeability.read more
Citations
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Cell surface P-glycoprotein associated with multidrug resistance in mammalian cell lines.
TL;DR: The plasma membranes of hamster, mouse, and human tumor cell lines that display multiple resistance to drugs were examined and increased expression of a 170,000-dalton surface antigen was found to be correlated with multidrug resistance.
Journal ArticleDOI
Active outward transport of daunomycin in resistant Ehrlich ascites tumor cells.
TL;DR: Investigation of the mechanism of a previously reported decreased accumulation of daunomycin in resistant compared to wild-type Ehrlich ascites tumor cells was investigated in vitro, and the effect suggests that these drugs are transported by the same extrusion mechanism as daunaomycin.
Journal ArticleDOI
Drug Transporters in the Central Nervous System: Brain Barriers and Brain Parenchyma Considerations
TL;DR: The novel localization of drug transporters in brain parenchyma cells, such as microglia and astrocytes, suggest a reconsideration of the present conceptualization of brain barriers as it relates to drug transport.
Journal ArticleDOI
Genetic and biochemical characterization of multidrug resistance
John R. Riordan,Victor Ling +1 more
TL;DR: Property of multidrug resistance mutants and their genetic characterizations are described and applications of such mutants for investigating aspects of membrane functions and drug transport are outlined.
Journal ArticleDOI
Amplification of specific DNA sequences correlates with multi-drug resistance in Chinese hamster cells.
TL;DR: It is shown that both Adriamycin- and colchicine-resistant cells contain amplified DNA fragments, some of which are amplified in both of these independently derived cell lines, and loss of the multi-drug resistance phenotype on growth in the absence of drugs correlates with the loss of amplified DNA.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Book ChapterDOI
Actinomycin and Nucleic Acid Function
E. Reich,Irving H. Goldberg +1 more
TL;DR: A model recently proposed for the reaction of actinomycin with DNA and to the implications of this model for the template function of helical nucleic acids for the role of RNA metabolism in many systems is related particularly to this discussion.
Journal ArticleDOI
Quantitative Estimation of Sialic Acids. III. An Anion Exchange Resin Method.
Journal Article
Uptake and Retention of Daunomycin by Mouse Leukemic Cells as Factors in Drug Response
TL;DR: Survival of mice bearing different transplantable tumors and treated with Daunomycin was compared with the capacity of the tumor cells for uptake of the drug in vitro and for drug uptake and retention in vivo to indicate that the ability of these cells to retain Daunaomycin in vivo was a determinant of drug response.
Journal ArticleDOI
Evaluation of 4-(2-hydroxyethyl)-1-piperazineëthanesulfonic acid (HEPES) as a tissue culture buffer.
TL;DR: HEPES had a minimal effect on the oxidation-reduction potential of well-poised media and had no effect on rubella virus titrations or on hemagglutination assays of polyoma or Sendai virus.
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