Journal ArticleDOI
On the one‐phase state of aqueous protein‐uncharged polymer systems: Casein‐guar gum system
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TLDR
In this paper, the effect of polymer-solvent and polymer-polymer interactions on the phase state of casein-guar aqueous systems was investigated and different solvent conditions were used to try to clarify the nature (electrostatic or nonelectrostatic) of the interaction between the two macromolecular species.Abstract:
Although the majority of biopolymers are incompatible in water, systems containing casein molecules and a neutral polysaccharide (guar gum galactomannan) showed phase separation only at an ionic strength above 0.09–0.2. Static light scattering, circular dichroism spectroscopy, velocity sedimentation, viscosimetry, phase analysis in different solvents, and Rosenberg's method were used to estimate the effect of polymer–solvent and polymer–polymer interactions on the phase state of casein-guar aqueous systems. Different solvent conditions were used to try to clarify the nature (electrostatic or nonelectrostatic) of the interaction between the two macromolecular species. Data obtained show that the dominant mechanism controlling the single-phase state at low ionic strength (below 0.01) involves the formation of water-soluble weak interpolymer complexes, which may be destroyed by increasing ionic strength. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 71: 471–482, 1999read more
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Journal ArticleDOI
Phase separation in aqueous gelatin–κ-carrageenan systems
Yu.A. Antonov,Maria P. Gonçalves +1 more
TL;DR: The phase diagram of the system, in the above mentioned conditions, indicates a possible specific interaction of the two biopolymers even at a very high ionic strength.
Journal ArticleDOI
Calorimetric and structural investigation of the interaction between bovine serum albumin and high molecular weight dextran in water.
TL;DR: This work studies specific interactions between a small globular protein and a highly flexible, branched polysaccharide using differential scanning calorimetry (DSC), circular dichroism (CD), fluorescence, and turbidimetry measurements.
Journal ArticleDOI
Characterization of galactomannans isolated from legume endosperms of Caesalpinioideae and Faboideae subfamilies by multidetection aqueous SEC
TL;DR: Galactomannans isolated from legume seed endosperms, including those of commercial interest, have been characterized by multidetection aqueous SEC, finding that prefactors in the [ η ]– M and R v – M relationships as well as the unperturbed parameter K Θ decrease in proportion to DG and therefore chain density varies within a narrow range for galactomans as a whole.
Journal ArticleDOI
Acid thinned jicama and maize starches as fat substitute in stirred yogurt
Silvia L. Amaya-Llano,Silvia L. Amaya-Llano,A.L. Martínez-Alegría,José de Jesús Zazueta-Morales,Fernando Martínez-Bustos +4 more
TL;DR: In this paper, the acid degradation of jicama and maize starches was not excessive as revealed by the positive blue value, amylose content, gel formation and gel thermo-reversibility.
Journal ArticleDOI
Structure formation and phase-separation behaviour of aqueous casein–alginate emulsions in the presence of strong polyelectrolyte
TL;DR: In this article, the effect of strong polyelectrolyte on the structure formation and phase-separation behavior in aqueous sodium caseinate-sodium alginate (WSC-SA) water-in-water emulsion with the droplet morphology in the concentration ranges both near and far from the critical point (CP).
References
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Reference BookDOI
Food Polysaccharides and Their Applications
TL;DR: Alistair M. Stephen and Shirley C. Cui as mentioned in this paper proposed a method for the detection of polysaccharides in foods, and showed that the method can be applied in the field of agriculture.
Journal ArticleDOI
Determination of protein secondary structure in solution by vacuum ultraviolet circular dichroism
S. Brahms,J.G. Brahms +1 more
TL;DR: By including all types of secondary structure and by enlarging substantially the spectral region, the method permits analysis of the conformation of a variety of proteins, such as nucleic acid-binding proteins, immunoglobulins and β-pleated sheet-rich proteins.
Journal ArticleDOI
Variable selection method improves the prediction of protein secondary structure from circular dichroism spectra.
TL;DR: Although the variable selection method improves the analysis of circular dichroism data truncated at 190 nm, data measured to 178 nm gives superior results, it is shown that improving the fit to the measured CD beyond the accuracy of the data can result in poorer analyses.