On the size of the DNA in the mammalian chromosome. Structural subunits.
J.T. Lett,E.S. Klucis,C. Sun +2 more
TLDR
The size of the subunit of single-stranded DNA is circa 5 x 10(8) daltons, and it is of sufficient length to contain a number of synthetic units, replicons as mentioned in this paper.About:
This article is published in Biophysical Journal.The article was published on 1970-03-01 and is currently open access. It has received 143 citations till now. The article focuses on the topics: Protein subunit & DNA.read more
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Isolation of high-molecular-weight DNA from mammalian cells.
TL;DR: A preparative method for isolating high-molecular-weight DNA from animal cells is described, based on the use of proteinase K, a powerful proteolytic enzyme with a broad action spectrum, which is very active in the presence of sodium dodecylsulfate and ethylene-diamine tetraacetate.
Journal ArticleDOI
Fractionation of DNA from mammalian cells by alkaline elution
TL;DR: The method of alkaline elution provides a sensitive measure of DNA single-strand length distribution in mamalian cells and is applicable to a variety of problems concerning DNA damage, repair, and replication.
Journal ArticleDOI
Postreplication repair of DNA in ultraviolet-irradiated mammalian cells
TL;DR: To determine whether the gaps in the daughter strands opposite the pyrimidine dimers are filled in with newly synthesized DNA by a recombinational mechanism or by de novo synthesis, irradiated cells were pulse-labeled with radioactive thymidine, followed by incubation in bromodeoxyuridine and exposure to light of wavelength 313 nm.
Journal ArticleDOI
Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis
TL;DR: This report describes a part of the evolution of microgel electrophoresis in the author's laboratory for the last 15 years and describes the importance of estimation of DNA single and double strand breaks, DNA crosslinks and apoptosis.
Journal ArticleDOI
A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides
TL;DR: The single-cell gel electrophoretic (SCG) technique for the in situ quantitation of DNA single-strand breaks and alkali-labile sites in cultured human-fibroblasts is standardized and applications for in vitro toxicology are discussed.
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