Oral immunization and protection of raccoons (Procyon lotor) with a vaccinia-rabies glycoprotein recombinant virus vaccine.
Charles E. Rupprecht,Tadeusz J. Wiktor,David H. Johnston,Amir N. Hamir,Bernhard Dietzschold,William H. Wunner,Lawrence T. Glickman,Hilary Koprowski +7 more
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This work demonstrates the feasibility of a recombinant virus vaccine containing the rabies glycoprotein gene for immunization of raccoons, and possibly other wildlife, to obtain long-term protection against rabies.Abstract:
Animal rabies control has been frustrated by the existence of multiple wildlife reservoirs and the lack of efficacious oral vaccines. In this investigation, raccoons fed a vaccinia-rabies glycoprotein recombinant virus in a sponge bait developed rabies virus-neutralizing antibody (0.6-54.0 units) and resisted street rabies virus infection 28 and 205 days after feeding. Additional raccoons immunized by oral infusion with attenuated antigenic variants of rabies virus strains CVS-11 and ERA failed to develop rabies virus-neutralizing antibody. This work demonstrates the feasibility of a recombinant virus vaccine containing the rabies glycoprotein gene for immunization of raccoons, and possibly other wildlife, to obtain long-term protection against rabies.read more
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Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety
TL;DR: A genetically altered vaccinia virus that is unable to replicate in mammalian cells and produces diminished cytopathic effects retains the capacity for high-level gene expression and immunogenicity while promising exceptional safety for laboratory workers and potential vaccine recipients.
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Vaccinia virus: a tool for research and vaccine development
TL;DR: Rec recombinant vaccinia virus is no longer needed for smallpox immunization, but now serves as a useful vector for expressing genes within the cytoplasm of eukaryotic cells.
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Predicting the spatial dynamics of rabies epidemics on heterogeneous landscapes
TL;DR: A stochastic spatial model of rabies spread through the state of Connecticut is developed and it is found that large rivers act as semipermeable barriers, leading to a 7-fold reduction in the local rates of propagation.
Journal ArticleDOI
Expression of the Rabies Virus Glycoprotein in Transgenic Tomatoes
Peter B. McGarvey,John Hammond,M. M. Dienelt,Douglas C. Hooper,Zhen Fang Fu,Bernhard Dietzschold,Hilary Koprowski,Frank H. Michaels +7 more
TL;DR: These transgenic plants should provide a valuable tool for the development of edible oral vaccines in light of the previous demonstration that orally administered rabies G-protein from the same ERA strain elicits protective immunity in animals.
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Expression in plants and immunogenicity of plant virus-based experimental rabies vaccine
Vidadi Yusibov,Douglas C. Hooper,Sergei Spitsin,Nina Fleysh,Rhonda B. Kean,Tatiana Mikheeva,Deepali Deka,Alexander V. Karasev,S Cox,J Randall,Hilary Koprowski +10 more
TL;DR: Three of five volunteers who had previously been immunized against rabies virus with a conventional vaccine specifically responded against the peptide antigen after ingesting spinach leaves infected with the recombinant virus, indicating the potential of the plant virus-based expression systems as supplementary oral booster for rabies vaccinations.
References
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Characterization of an antigenic determinant of the glycoprotein that correlates with pathogenicity of rabies virus
Bernhard Dietzschold,William H. Wunner,Tadeusz J. Wiktor,A D Lopes,M. Lafon,C L Smith,Hilary Koprowski +6 more
TL;DR: It is concluded that arginine-333 is essential for the integrity of an antigenic determinant and for the ability of rabies viruses to produce lethal infection in adult mice.
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Expression of rabies virus glycoprotein from a recombinant vaccinia virus
Marie-Paule Kieny,Richard Lathe,Robert Drillien,Danièle Spehner,S. Skory,D. Schmitt,T. Wiktor,Hilary Koprowski,J P Lecocq +8 more
TL;DR: This work altered the rabies glycoprotein cDNA by site-directed mutagenesis and removed the poly(dG) tail, aligned the modified cDNA with an early VV promoter sequence inserted within a cloned copy of the vaccinia thymidine kinase gene and transfected this plasmid into VV-infected cells to produce a novel rabies vaccine.
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Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene
Tadeusz J. Wiktor,R I Macfarlan,K J Reagan,Bernhard Dietzschold,P J Curtis,William H. Wunner,Marie-Paule Kieny,Richard Lathe,J P Lecocq,M Mackett +9 more
TL;DR: V-RGpro8 virus was highly effective in priming mice to generate a secondary rabies virus-specific cytotoxic T-lymphocyte response following culture of lymphocytes with either ERA or PM strains of rabiesirus.
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Oral vaccination of foxes against rabies
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Antigenic variants of rabies virus.
TL;DR: This study only allowed us to recognize three groups of viruses with different nucleocapsid patterns, and no difference, as far as protection with a Pitmann-Moore vaccine is concerned, between four of these strains.