Journal ArticleDOI
Organization of the 5S ribosomal RNA genes in the genome of tomato
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TLDR
A detailed characterization of the 5S rRNA genes of tomato with respect to chromosome assignment, chromosomal localization, copy number, and physical size is reported.Abstract:
The 5S ribosomal RNA (rRNA) genes represent one of the most abundant gene families in eukaryotic genomes and have been a model system for the study of molecular organization and evolution of multigene families in eukaryotes. This paper reports a detailed characterization of the 5S rRNA genes of tomato (Lycopersicon esculentum) with respect to chromosome assignment, chromosomal localization, copy number, and physical size. By restriction fragment length polymorphism, the tandemly repeated 5S rRNA genes were assigned to a region of chromosome 1 of tomato. These results were confirmed by in situ hybridization onto tomato metaphase chromosomes. The single hybridization signal was localized to the short arm of chromosome 1, in a region close to the centromere. Based on reconstruction experiments, it was estimated that the 400-bp repeating unit occurs in approximately 1000 copies per haploid genome. Physical characterization of the entire locus was then performed by means of pulsed-field gel electrophoresis. Di...read more
Citations
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Journal ArticleDOI
High density molecular linkage maps of the tomato and potato genomes.
Steven D. Tanksley,Martin W. Ganal,James P. Prince,M. C. de Vicente,M. W. Bonierbale,Pierre Broun,T. M. Fulton,James J. Giovannoni,Silvana Grandillo,Gregory B. Martin +9 more
TL;DR: Currently tomato and potato are among the most thoroughly mapped eukaryotic species and the availability of high density molecular linkage maps should facilitate chromosome walking, quantitative trait mapping, marker-assisted breeding and evolutionary studies in these two important and well studied crop species.
Journal ArticleDOI
Nonisotopic in situ hybridization and plant genome mapping: the first 10 years.
Jiming Jiang,Bikram S. Gill +1 more
TL;DR: A critical review is made on the present resolution of the ISH technique and the future outlook of ISH research is discussed.
Journal ArticleDOI
Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato
TL;DR: It is demonstrated that fluorescence in situ hybridization (FISH) signals derived from bacterial artificial chromosomes (BACs) can be used as chromosome-specific cytogenetic DNA markers for chromosome identification in potato.
Journal ArticleDOI
Physical mapping of rRNA genes by fluorescent in-situ hybridization and structural analysis of 5S rRNA genes and intergenic spacer sequences in sugar beet (Beta vulgaris).
TL;DR: The use of fluorescent in-situ hybridization, complemented by molecular data, for gene mapping and for integrating genetic and physical maps of beet species is discussed.
Journal ArticleDOI
Genetic analysis of RFLPs, GATA microsatellites and RAPDs in a cross between L. esculentum and L. pimpinellifolium.
Silvana Grandillo,Steve Tanksley +1 more
TL;DR: A population of 257 BC1 plants was developed from a cross between an elite processing line of tomato (Lycopersicon esculentum cv‘M82-1-7’) and the closely related wild species L. pimpinellifolium (LA1589) to construct a genetic linkage map suitable for QTL analysis to be conducted in different backcross generations.
References
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Journal ArticleDOI
MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations
Eric S. Lander,Eric S. Lander,Philip Green,Jeff Abrahamson,Aaron Barlow,Mark J. Daly,Stephen E Lincoln,Lee Newburg +7 more
TL;DR: A computer package, called MAPMAKER, designed specifically for the construction of linkage maps in a number of organisms, including the human and several plants, and it is outlined the mapping strategies that have been used.
Journal ArticleDOI
Rapid flow cytometric analysis of the cell cycle in intact plant tissues.
David W. Galbraith,Kristi R. Harkins,Joyce M. Maddox,Nicola M. Ayres,Dharam P. Sharma,Ebrahim Firoozabady +5 more
TL;DR: The amount of nuclear DNA in the homogenates of monocotyledonous and dicotylingonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.
Journal ArticleDOI
Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes
W. L. Gerlach,T.A. Dyer +1 more
TL;DR: Examples of both the 410 and 500 bp size classes of repeating units containing wheat 5S rRNA genes have been cloned in plasmid pBR322 and sequenced and the structural genes showed sequence microheterogeneity.
Journal ArticleDOI
Genetics of actin-related sequences in tomato.
R. Bernatzky,Steven D. Tanksley +1 more
TL;DR: The results suggest that the number of actin loci has not been stable during the evolution of dicots, and a number of these mapped loci lie in regions of the genome previously devoid of molecular markers and thus may be useful in basic and applied genetic research.
Journal ArticleDOI
Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
TL;DR: Two techniques were especially useful in the mapping: "cloning" 14S + 25S DNA on the plasmid pMB9 to amplify individual R1 fragments, and digesting DNA with R1 in the presence of the antibiotic distamycin A to produce specific partial digestion products.
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