Orthogonally-tunable and ER-targeting fluorophores detect avian influenza virus early infection
Taewon Kang,Md. Mamunul Haque,Bo-Hee Lee,Kyung Tae Hong,Seong Cheol Hong,Younghun Kim,Jesang Lee,Jun-Seok Lee,Dongwhan Lee +8 more
TLDR
In this paper , the authors report stimuli-responsive light-emitters built on a T-shaped benzimidazole platform, and consecutive borylation reactions to produce a library of homologs displaying systematic changes in fluorescence quantum yield and environmental sensitivity.Abstract:
Abstract Cell-based assays can monitor virus infection at a single-cell level with high sensitivity and cost-efficiency. For this purpose, it is crucial to develop molecular probes that respond selectively to physiological changes in live cells. We report stimuli-responsive light-emitters built on a T-shaped benzimidazole platform, and consecutive borylation reactions to produce a library of homologs displaying systematic changes in fluorescence quantum yield and environmental sensitivity. We find that certain fluorophores localize selectively at the endoplasmic reticulum, and interact with proteins involved in the stress signaling pathways. Notably, the mono-borylated compound responds selectively to the stress conditions by enhancing fluorescence, and detects avian influenza virus infection at the single-cell level. Our findings demonstrate the unprecedented practical utility of the stress-responsive molecular probes to differentiate cellular states for early diagnosis. read more
Citations
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Recent advances in organic near-infrared ratiometric small-molecule fluorescent probes
TL;DR: In this paper , the authors systematically summarized the recent advances (2020-2022) in these kinds of probes and discussed the design strategies, sensing mechanisms, and bioapplications of these intelligent fluorescent probes.
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Single‐Benzene Dual‐Emitters Harness Excited‐State Antiaromaticity for White Light Generation and Fluorescence Imaging
Sanna Tuomela,Team, TSDC +1 more
TL;DR: In this article , dual-emissive single-benzene fluorophores (SBFs) were synthesized with a record high solubility in perfluorocarbon solvents.
Journal ArticleDOI
Single-Benzene Dual-Emitters Harness Excited-State Antiaromaticity for White Light Generation and Fluorescence Imaging.
TL;DR: In this paper , dual-emissive single-benzene fluorophores (SBFs) were synthesized with a record high solubility in perfluorocarbon solvents.
References
More filters
Journal ArticleDOI
Quantum mechanical continuum solvation models.
TL;DR: This paper presents a meta-modelling procedure called "Continuum Methods within MD and MC Simulations 3072", which automates the very labor-intensive and therefore time-heavy and expensive process of integrating discrete and continuous components into a discrete-time model.
Journal ArticleDOI
Oligomerization and phosphorylation of the Ire1p kinase during intracellular signaling from the endoplasmic reticulum to the nucleus.
Caroline E. Shamu,Peter Walter +1 more
TL;DR: Molecular genetic and biochemical studies described here suggest that, as in the case of growth factor receptors of higher eukaryotic cells, Ire1p oligomerizes in response to the accumulation of unfolded proteins in the ER and is phosphorylated in trans by otherIre1p molecules as a result of oligomerization.
Journal ArticleDOI
A survey of Hammett substituent constants and resonance and field parameters
Corwin Hansch,A. Leo,R. W. Taft +2 more
TL;DR: The Hammett equation has been widely used for the study and interpretation of organic reactions and their mechanisms as mentioned in this paper, and it is astonishing that u constants, obtained simply from the ionization of organic acids in solution, can frequently predict successfully equilibrium and rate constants for a variety of families of reactions in solution.
Journal ArticleDOI
Solvatochromic Dyes as Solvent Polarity Indicators
TL;DR: In this paper, a review compiles positively and negatively solvatochromic compounds which have been used to establish empirical scales of solvent polarity by means of UV/vis/near-IR spectroscopic measurements in solution.
Journal ArticleDOI
XBP1 mRNA Is Induced by ATF6 and Spliced by IRE1 in Response to ER Stress to Produce a Highly Active Transcription Factor
TL;DR: The transcription factor XBP1, a target of ATF6, is identified as a mammalian substrate of such an unconventional mRNA splicing system and it is shown that only the spliced form of X BP1 can activate the UPR efficiently.