Phosducin, potential role in modulation of olfactory signaling
Ingrid Boekhoff,Kazushige Touhara,Stefan Danner,James Inglese,Martin J. Lohse,Heinz Breer,Robert J. Lefkowitz +6 more
TLDR
The results suggest that phosducin may act as a phosphorylation-dependent switch in second messenger signaling cascades, regulating the kinetics of desensitization processes by controlling the activity of Gβγ-dependent GRKs.About:
This article is published in Journal of Biological Chemistry.The article was published on 1997-02-14 and is currently open access. It has received 41 citations till now. The article focuses on the topics: Phosducin & Protein kinase A.read more
Citations
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Molecular and Cellular Basis of Addiction
TL;DR: Current research needs to understand the types of adaptations that underlie the particularly long-lived aspects of addiction, such as drug craving and relapse, and to identify specific genes that contribute to individual differences in vulnerability to addiction.
Journal ArticleDOI
G protein-coupled receptor kinases.
TL;DR: This review focuses on the regulation of GRK activity by a variety of allosteric and other factors: agonist-stimulated GPCRs, beta gamma subunits of heterotrimeric GTP- binding proteins, phospholipid cofactors, the calcium-binding proteins calmodulin and recoverin, posttranslational isoprenylation and palmitoylation, autophosphorylation, and protein kinase C-mediated GRK phosphorylation.
Journal ArticleDOI
Molecular mechanisms and regulation of insulin exocytosis as a paradigm of endocrine secretion
TL;DR: Insights into the progression of the exocytotic vesicle from docking to fusion and how these processes are precisely regulated by proteins and second messengers may provide the basis for new therapeutic principles.
Journal ArticleDOI
Molecular mechanisms of opiate and cocaine addiction.
TL;DR: This work has focused on a role for transcription factors, and the associated alterations in gene expression, in mediating part of this long-lasting, drug-induced molecular and behavioral plasticity in animal models of addiction.
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The variable and conserved interfaces of modeled olfactory receptor proteins.
Yitzhak Pilpel,Doron Lancet +1 more
TL;DR: It is proposed that a set of 17 hypervariable residues, which point to the barrel interior and are more extracellularly disposed, constitute the odorant complementarity determining regions and form an olfactory‐unique aspect of the binding pocket.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Role of beta gamma subunits of G proteins in targeting the beta-adrenergic receptor kinase to membrane-bound receptors.
Julie A. Pitcher,James Inglese,Joyce B. Higgins,Jeffery L. Arriza,Patrick J. Casey,Chong Kim,Jeffery L. Benovic,Madan M. Kwatra,Marc G. Caron,Robert J. Lefkowitz +9 more
TL;DR: The rate and extent of the agonist-dependent phosphorylation of beta 2-adrenergic receptors and rhodopsin by Beta ARK are markedly enhanced on addition of G protein beta gamma subunits, suggesting that the enzyme preferentially binds specific beta gamma complexes.
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Binding of G protein beta gamma-subunits to pleckstrin homology domains.
TL;DR: It is demonstrated that glutathione S-transferase-fusion proteins, containing sequences encompassing the PH domain of nine proteins from this group, bind G beta gamma to varying extents, indicating that protein-protein interactions between G beta Gamma and PH domain-containing proteins may play a significant role in cellular signaling analogous to that previously demonstrated for Src homology 2 and 3 domains.
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Rapid kinetics of second messenger formation in olfactory transduction
TL;DR: An odorant-induced, transient accumulation of cyclic AMP in isolated olfactory cilia from rats, and the generation of inositol trisphosphate in antennal preparations from insects are reported, both of which show subsecond time courses that are sufficiently rapid to mediate the odorants-regulated permeability of o aroma receptor cells.