Phosphate pool dynamics in the arbuscular mycorrhizal fungus Glomus intraradices studied by in vivo31P NMR spectroscopy
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TLDR
The hypothesis that polyP is the major P species translocated in the tubular vacuolar network, the presence of which was previously demonstrated in AM fungi, is supported.Abstract:
Summary
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Polyphosphate (polyP) is presumably central to phosphate (P) metabolism of arbuscular mycorrhizal (AM) fungi, but its synthesis, location and chain lengths are poorly characterized. Here, we applied noninvasive and nondestructive nuclear magnetic resonance (NMR) spectroscopy to obtain novel information on AM fungal polyP.
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In vivo31P NMR spectroscopy was used to characterize polyP and other P pools in external hyphae and in mycorrhizal roots of associations between Glomus intraradices and cucumber (Cucumis sativus).
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A time-course study of P-starved external hyphae supplied with additional P showed that polyP appeared more rapidly than vacuolar inorganic P. These P metabolites also appeared in the roots, but later. PolyP considerably exceeded amounts of vacuolar inorganic P, where it was located in acidic, presumably vacuolar compartments, and had a short average chain length.
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The rapid synthesis of polyP might be important for the maintenance of effective hyphal P uptake. Our data support the hypothesis that polyP is the major P species translocated in the tubular vacuolar network, the presence of which was previously demonstrated in AM fungi.read more
Citations
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Journal ArticleDOI
Roles of Arbuscular Mycorrhizas in Plant Nutrition and Growth: New Paradigms from Cellular to Ecosystem Scales
Sally E. Smith,F. Andrew Smith +1 more
TL;DR: New physiological and molecular evidence shows that for phosphorus the mycorrhizal pathway (MP) is operational regardless of plant growth responses (positive or negative) and has important implications for consideration of AM symbioses in ecological, agronomic, and evolutionary contexts.
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A Medicago truncatula phosphate transporter indispensable for the arbuscular mycorrhizal symbiosis.
TL;DR: It is shown that MtPT4, a Medicago truncatula member of subfamily I, is essential for the acquisition of Pi delivered by the AM fungus, which leads to premature death of the arbuscules; the fungus is unable to proliferate within the root, and symbiosis is terminated.
Journal ArticleDOI
Symbiotic phosphate transport in arbuscular mycorrhizas
TL;DR: The study of phosphate transport mechanisms and their gene regulation will further the understanding of the intimate interaction between the two symbiotic partners.
Journal ArticleDOI
The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices (DAOM 197198) reveals functional tradeoffs in an obligate symbiont
Emilie Tisserant,Annegret Kohler,P. Dozolme-Seddas,Raffaella Balestrini,Karim Benabdellah,Alexandre Colard,Alexandre Colard,Daniel Croll,Daniel Croll,C. da Silva,S. K. Gomez,Raman Koul,Nuria Ferrol,Valentina Fiorilli,Damien Formey,Philipp Franken,Nicole Helber,Mohamed Hijri,Luisa Lanfranco,Erika Lindquist,Y. Liu,Mathilde Malbreil,Emmanuelle Morin,Julie Poulain,Harris Shapiro,D. van Tuinen,A. Waschke,Concepción Azcón-Aguilar,Guillaume Bécard,Paola Bonfante,Maria J. Harrison,Helge Küster,Peter J. Lammers,Uta Paszkowski,Natalia Requena,Stefan A. Rensing,Christophe Roux,Ian R. Sanders,Yair Shachar-Hill,Gerald A. Tuskan,J. P. W. Young,Vivienne Gianinazzi-Pearson,Francis Martin +42 more
TL;DR: The first genome-wide analysis of the transcriptome from Glomus intraradices is reported, suggesting that the lack of a known sexual cycle in G. intrarodices is not a result of major deletions of genes essential for sexual reproduction and meiosis.
Journal ArticleDOI
The characterization of novel mycorrhiza-specific phosphate transporters from Lycopersicon esculentum and Solanum tuberosum uncovers functional redundancy in symbiotic phosphate transport in solanaceous species
Réka Nagy,Vladimir Karandashov,Veronique Chague,Katsiaryna Kalinkevich,M'Barek Tamasloukht,Guohua Xu,Iver Jakobsen,Avraham A. Levy,Nikolaus Amrhein,Marcel Bucher +9 more
TL;DR: The first mycorrhiza-specific plant Pi transporter was StPT3 from potato and its orthologue StPT4 from potato, both being members of the Pht1 family of plant Pi transporters.
References
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Journal ArticleDOI
A modified single solution method for the determination of phosphate in natural waters
J. Murphy,J.P. Riley +1 more
TL;DR: In this article, a single solution reagent was described for the determination of phosphorus in sea water, which consists of an acidified solution of ammonium molybdate containing ascorbic acid and a small amount of antimony.
Journal ArticleDOI
An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots
TL;DR: The standard error of four methods of assessment based on observations of stained root samples either randomly arranged in a petri dish or mounted on microscope slides are calculated.
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