Journal ArticleDOI
Polarized sorting in epithelia
Kai Simons,Angela Wandinger-Ness +1 more
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This article is published in Cell.The article was published on 1990-07-27. It has received 524 citations till now. The article focuses on the topics: Polarity (physics) & Polarite.read more
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Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface
Deborah A. Brown,John K. Rose +1 more
TL;DR: It is shown that a protein with a glycosylphosphatidyl inositol (GPI) anchor can be recovered from lysates of epithelial cells in a low density, detergent-insoluble form, supporting the model proposed by Simons and colleagues for sorting of certain membrane proteins to the apical surface after intracellular association with glycosphingolipids.
Journal ArticleDOI
A knowledge base for predicting protein localization sites in eukaryotic cells
Kenta Nakai,Minoru Kanehisa +1 more
TL;DR: An expert system is reported for predicting localization sites of proteins only from the information on the amino acid sequence and the source origin, which is powerful and flexible enough to be used in genome analyses.
Journal ArticleDOI
Signal transducing molecules and glycosyl-phosphatidylinositol-linked proteins form a caveolin-rich insoluble complex in MDCK cells
TL;DR: It is proposed that caveolin could function as a transmembrane adaptor molecule that couples luminal G PI-linked proteins with cytoplasmically oriented signaling molecules during GPI- Membrane trafficking or GPI -mediated signal transduction events.
Journal ArticleDOI
Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors
TL;DR: This work uses quantitative high-resolution MS to specifically detect proteins depleted from rafts by cholesterol-disrupting drugs, resulting in a set of 241 authentic lipid raft components, providing the first large-scale and unbiased evidence for the connection of rafts with signaling and place limits on the fraction of plasma membrane composed by rafts.
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Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of drosophila
TL;DR: Evidence is presented to show that the insertion of crumbs into the plasma membrane is necessary and sufficient to confer apical character on a membrane domain, and to suggest that crumbs plays a key role in specifying the apical plasma membrane domain of ectodermal epithelial cells of Drosophila.
References
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Journal ArticleDOI
The Biogenesis of Lysosomes
Stuart Kornfeld,Ira Mellman +1 more
TL;DR: Article de synthese sur les lysosomes: biogenese, definition, role biologique, Les enzymes lysOSomales sont decrites ainsi that leur role dans l'exo et l'endocytose.
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Lipid sorting in epithelial cells.
Kai Simons,G. van Meer +1 more
TL;DR: Lipid biochemistry has remained a fairly esoteric branch of molecular cell biology, but this situation is now gradually changing with the discovery of phosphoinositide involvement in signal transduction.
Journal ArticleDOI
Morphogenesis of the polarized epithelial cell phenotype
TL;DR: Polarized epithelial cells play fundamental roles in the ontogeny and function of a variety of tissues and organs in mammals and are the first overt sign of cellular differentiation in early embryonic development.
Journal ArticleDOI
Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi.
Journal ArticleDOI
Cell Surface Polarity in Epithelia
Kai Simons,S D Fuller +1 more
TL;DR: Systemes experimentaux pour etudier la biogenese de la surface cellulaire, organisation moleculaire de the surface de the cellule epitheliale, d'endocytose et transcytose, et insertion des proteines membranaires.
Related Papers (5)
Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface
Deborah A. Brown,John K. Rose +1 more