Journal ArticleDOI
Polyoxometalate binding to human serum albumin: a thermodynamic and spectroscopic approach.
Guangjin Zhang,Bineta Keita,Constantin T. Craescu,Simona Miron,and Pedro de Oliveira,Louis Nadjo +5 more
TLDR
The present work reveals a protein conformation change upon P5W30 binding, a new feature not explicitly documented in previous studies, which is a simple exothermic process, with several binding sites.Abstract:
The molecular recognition of polyoxometalates by human serum albumin is studied using two different polyoxometalates (POMs) at pH 7.5. The results are compared with those obtained at pH 3.5 and 9.0. At pH 7.5, both POMs strongly interact with the protein with different binding behaviors. The Keggin shaped POM, [H2W12O40]6- (H2W12), specifically binds the protein, forming a complex with a 1:1 stoichiometry with Ka = 2.9 × 106 M-1. The binding constant decreased dramatically with the increase of the ionic strength, thus indicating a mostly electrostatic binding process. Isothermal titration calorimetry (ITC) experiments show that the binding is an enthalpically driven exothermic process. For the wheel shaped POM [NaP5W30O110]14- (P5W30), there are up to five binding sites on the protein. Increasing the ionic strength changes the binding behavior significantly, leading to a simple exothermic process, with several binding sites. Competitive binding experiments indicate that the two POMs share one common bindi...read more
Citations
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Polyoxometalates: Building Blocks for Functional Nanoscale Systems
TL;DR: The possibilities of creating highly sophisticated functional hierarchical systems with multiple, interdependent, functionalities along with a critical analysis that allows the non-specialist to learn the salient features of POMs are discussed.
Journal ArticleDOI
Hybrid Organic−Inorganic Polyoxometalate Compounds: From Structural Diversity to Applications
TL;DR: Polyoxometalates (POMs) are discrete anionic metaloxygen clusters which can be regarded as soluble oxide fragments which play a great role in various areas ranging from catalysis, medicine, electrochemistry, photochromism,5 to magnetism.
Journal ArticleDOI
Recent advances on polyoxometalate-based molecular and composite materials
Yu-Fei Song,Ryo Tsunashima +1 more
TL;DR: This critical review summarizes recent progress on POM-based molecular and composite materials, and particularly highlights the emerging areas that are closely related to surface, electronic, energy, environment, life science, etc.
Journal ArticleDOI
Polyoxometallate als Bausteine für funktionelle Nanosysteme
TL;DR: In this article, a periodensystem der Polyoxometallat-Baueinheiten (POM) is presented, in which a number of aktuelle Herausforderungen beim design funktioneller Systeme auf der Basis of POM-Baubien are discussed.
Journal ArticleDOI
The use of polyoxometalates in protein crystallography - An attempt to widen a well-known bottleneck.
TL;DR: Polyoxometalates (POMs) are discrete polynuclear metal-oxo anions with a fascinating variety of structures and unique chemical and physical properties as mentioned in this paper.
References
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Journal ArticleDOI
Catalysis by Heteropoly Acids and Multicomponent Polyoxometalates in Liquid-Phase Reactions
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Polyoxometalates in Medicine
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Photo- and Electrochromism of Polyoxometalates and Related Materials
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Conformational transitions of the three recombinant domains of human serum albumin depending on pH.
TL;DR: Evidence is provided that the loosening of the HSA structure in the N-F transition takes place primarily in HSA-DOM III and that HSA -DOM I undergoes a structural rearrangement with only minor changes in secondary structure, whereas Hsa-DOM II transforms to a molten globule-like state as the pH is reduced.
Journal ArticleDOI
A double-sided radio jet from the compact Galactic Centre annihilator 1E1740.7-2942
TL;DR: In this paper, the authors observed the radio structure of the hard X-ray source 1E1740.7-2942 using the Very Large Array and found that its radio structure is that of a doublesided jet emanating from a compact and variable core.