Journal ArticleDOI
Preliminary Evaluation of the Major Protocol Variables for the Hershberger Castrated Male Rat Assay for the Detection of Androgens, Antiandrogens, and Metabolic Modulators
J. Ashby,P.A. Lefevre +1 more
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TLDR
The Hershberger assay appears to be a sensitive and practical test system for the detection of androgens, antiandrogens, and inhibitors of male sex hormone anabolism, however, continued attention will need to be given to the derivation of a standard protocol for the routine deployment of the assay.About:
This article is published in Regulatory Toxicology and Pharmacology.The article was published on 2000-02-01. It has received 37 citations till now. The article focuses on the topics: Antiandrogens.read more
Citations
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Journal ArticleDOI
Evaluation of a 15-Day Screening Assay Using Intact Male Rats for Identifying Antiandrogens
TL;DR: 5 of the six test substances were identified as endocrine-active substances consistent with their known/proposed mechanism(s) of action and increased relative liver weight, illustrating that the ability to identify EACs using the intact male assay will be equivalent regardless of the route of compound administration.
Journal ArticleDOI
Evaluation of anti-androgenic activity of di-(2-ethylhexyl)phthalate.
Thomas Stroheker,Nicolas Cabaton,Gorian Nourdin,Jean-François Regnier,Jean-Claude Lhuguenot,Marie-Christine Chagnon +5 more
TL;DR: DEHP appeared not to be a 5alpha-reductase inhibitor and acted in an independent mechanism from the testicular production in the young rat and its major metabolites were anti-androgenic in vitro.
Journal ArticleDOI
Chlropyrifos-methyl shows anti-androgenic activity without estrogenic activity in rats.
TL;DR: CPM dose does not show estrogenic and anti-estrogenic activity in immature female rats, but it represents anti-androgensic activity by inhibition of the TP-stimulated increase of the weight of accessory sex organs.
Journal ArticleDOI
Proposed Mode of Action for In Utero Effects of Some Phthalate Esters on the Developing Male Reproductive Tract
TL;DR: The data indicate that C4–C6 phthalate esters inhibit processes in the Leydig cell, such as the synthesis of testosterone (T) and production of insulin-like factor 3 (insl3), both of which are required for normal development of male genitalia.
Book ChapterDOI
High-throughput screening for analysis of in vitro toxicity
TL;DR: The influence of combinatorial chemistry and high-throughput screening (HTS) technologies in the pharmaceutical industry during the last 10 years has been enormous, however, the attrition rate of drugs in the clinic due to toxicity during this period still remained 40-50%.
References
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Journal ArticleDOI
Myotrophic activity of 19-nortestosterone and other steroids determined by modified levator ani muscle method.
TL;DR: Preliminary steroid screening results indicate that 19-nortestosterone and other 19-nor analogs of androgens promise to be effective protein-anabolic, and relatively weak androgenic agents.
Journal ArticleDOI
Vinclozolin and p,p'-DDE alter androgen-dependent gene expression : in vivo confirmation of an androgen receptor-mediated mechanism
TL;DR: Data indicate that vinclozolin and p,p'-DDE act as antiandrogenic developmental effects in vivo by altering the expression of androgen-dependent genes.
Journal ArticleDOI
Environmental antiandrogens: developmental effects, molecular mechanisms, and clinical implications.
Kelce Wr,Elizabeth M. Wilson +1 more
TL;DR: In light of increasing concern over the potential endocrine disrupting effects of environmental pollutants, an in vitro/in vivo investigational strategy is presented which has proved useful in identifying chemicals with antiandrogen activity and their mechanism of action.
Journal ArticleDOI
The rodent uterotrophic assay: Critical protocol features, studies with nonyl phenols, and comparison with a yeast estrogenicity assay
J. Odum,P. A. Lefevre,S Tittensor,D. Paton,Edwin J. Routledge,Nicola Beresford,John P. Sumpter,John Ashby +7 more
TL;DR: It is concluded that three daily oral administrations of test chemicals to 21- to 22-day-old rats, followed by determination of absolute uterus weights on the fourth day, provide a sensitive and toxicologically relevant in vivo estrogenicity assay.