Journal ArticleDOI
Preparation of highly active (Na+ + K+)-ATPase from the outer medulla of rabbit kidney
TLDR
(Na + + K + )-ATPase from the outer medulla of rabbit kidney was purified to a specific activity of 881 ± 25 moles Pi/mg protein per hr at 37 C.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1969-09-24. It has received 143 citations till now. The article focuses on the topics: Na+/K+-ATPase & Differential centrifugation.read more
Citations
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Purification and characterization of (Na+ + K+)-ATPase III. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate
TL;DR: Analysis of the protein composition by sodium dodecylsulphate gel electrophoresis and determination of the capacities for binding of ATP and ouabain and for sodium-dependent phosphorylation show that the procedures lead to a true purification of the enzyme.
Journal ArticleDOI
The Na,K-ATPase.
TL;DR: Attention will be given to the biochemical characterization of the reaction mechanism underlying the coupling between hydrolysis of the substate ATP and transport of Na+ and K+.
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Mechanism of the Na+, K+ pump protein structure and conformations of the pure (Na+ + K+)-ATPase
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The sodium-potassium adenosinetriphosphatase.
TL;DR: The Phosphorylated Peptide Component of Purified NaK A TPase Preparations and the Reversal of the Reaction are presented, which show the importance of knowing the carrier and removal status of the carrier molecule.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Enzymatic basis for active transport of na+ and k+ across cell membrane.
TL;DR: Afhnity for Monovalent Cations and Quantitative Relation between Effect of Na+ + K+ on Enzyme System and Active Transport in Intact Cell.
Journal ArticleDOI
High-Resolution Density Gradient Sedimentation Analysis
TL;DR: The principle of stability for a sample layered in a density-gradient liquid column is discussed, and a method for separating ribonucleoprotein particles by means of sedimentation in the ultracentrifuge is described.