Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital
TLDR
The present prospective study found that definitive identification of ESBL genes is only possible by molecular detection methods and Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes.Abstract:
Introduction: Extended-spectrum beta-lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as penicillins, cephalosporins, and monobactams. They are derived from the narrow-spectrum beta-lactamases (TEM-1, TEM-2, or SHV-1) by mutations that alter the amino acid configuration around the enzyme active site. Aim: To determine the prevalence of ESBL (bla TEM , bla CTX-M , and bla SHV ) genes among the members of Enterobacteriaceae. Methodology: The present prospective study was carried out from January 2015 to June 2015 in the Department of Microbiology and Molecular Medicine of a Teaching Tertiary Care Hospital. A total of 526 urine samples were studied. Seventy-eight isolates were subjected to polymerase chain reaction for detection of ESBL genes. Results: In our study, ESBL genes were detected among 18 (45%) phenotypically confirmed ESBL producers and 20 (52.5%) phenotypically confirmed non-ESBL producers. The gene that predominated was bla TEM (48.7%), followed by bla CTX-M (7.6%) and bla SHV (5.1%). Conclusion: Definitive identification of ESBL genes is only possible by molecular detection methods. Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes.read more
Citations
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Journal ArticleDOI
Epidemiology of β-Lactamase-Producing Pathogens.
Karen Bush,Patricia A. Bradford +1 more
TL;DR: As selective pressure from β-lactam use continues, multiple β-Lactamases per organism are increasingly common, including pathogens carrying three different carbapenemase genes, warranting close attention to increased infection control measures and stewardship of the β- lactam-containing drugs in an effort to control selection of even more deleterious pathogens.
Journal ArticleDOI
Prevalence of TEM, SHV, and CTX-M genes of extended-spectrum β-lactamase-producing Escherichia coli strains isolated from urinary tract infections in adults.
TL;DR: The prevalence of MDR (multidrug resistance) ESBL-producing E. coli appears to be high and the highest identified gene was blaTEM, which can help physician’s select suitable empirical antibiotic regimens, so that antibiotics showing high-resistance pattern can be avoided.
Journal ArticleDOI
Distribution of bla CTX − M , bla TEM , bla SHV and bla OXA genes in Extended-spectrum- β -lactamase-producing Clinical isolates: A three-year multi-center study from Lahore, Pakistan
TL;DR: Prevalence of ESBL-producing isolates has increased 1.13 folds during study years, while distribution of the key genetic determinants of Extended-spectrum-β-lactamase-producing Clinical isolates in Pakistan has remained the same.
Journal ArticleDOI
Antibiotic resistance of pathogenic Acinetobacter species and emerging combination therapy
Bora Shin,Woojun Park +1 more
TL;DR: The classic mechanisms of Acinetobacter AMR are summarized, along with newly-discovered genetic AMR factors and currently available antimicrobial adjuvants that can enhance drug efficacy in the treatment of A. baumannii infections.
Journal ArticleDOI
Carbapenem-Resistant KPC- And TEM-Producing Escherichia coli ST131 Isolated from a Hospitalized Patient with Urinary Tract Infection: First Isolation in Molise Region, Central Italy, July 2018
Giancarlo Ripabelli,Michela Lucia Sammarco,Massimiliano Scutellà,Valentina Felice,Manuela Tamburro +4 more
TL;DR: This observation raises great concerns about the spread of carbapenem resistance in national and local settings with high endemicity level of KPC in K. pneumoniae, and underlines the importance of strengthening a proactive surveillance.
References
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Journal ArticleDOI
Antibiotic susceptibility testing by a standardized single disk method.
TL;DR: Recommendations of the National Committee for Clinical Laboratory Standards continue to be based on this publication; the “Kirby-Bauer” method is, among the many disk methods used in other countries, still the one that has been researched most thoroughly and updated continuously.
Book
Medical Laboratory Manual for Tropical Countries
TL;DR: Reading medical laboratory manual for tropical countries is also a way as one of the collective books that gives many advantages.
Journal ArticleDOI
Extended-Spectrum-β-Lactamase, AmpC, and Carbapenemase Issues
TL;DR: This Commentary focuses on the detection of ESBL resistance in gram-negative pathogens, the most important countermeasure against multidrug-resistant gram- negative pathogens in the absence of new, effective anti-gram-negative antibiotics.
Journal ArticleDOI
Simple and reliable multiplex PCR assay for detection of blaTEM, blaSHV and blaOXA-1 genes in Enterobacteriaceae
TL;DR: A rapid and simple multiplex polymerase chain reaction (PCR) which discriminates bla(TEM), bla (SHV) and bla-1 genes by generating fragments of 516, 392 and 619 bp respectively is developed.
Journal ArticleDOI
The CTX-M Conundrum: Dissemination of Plasmids and Escherichia coli Clones
Umaer Naseer,Arnfinn Sundsfjord +1 more
TL;DR: A global epidemic of bla(CTX-M)-encoding E. coli strains and plasmids are witnessing a global epidemic, which require serious attention and efficient infection control measures.