PROCUSTE1 Encodes a Cellulose Synthase Required for Normal Cell Elongation Specifically in Roots and Dark-Grown Hypocotyls of Arabidopsis
Mathilde Fagard,Thierry Desnos,Thierry Desprez,Florence Goubet,Guislaine Refrégier,Grégory Mouille,Maureen C. McCann,Catherine Rayon,Samantha Vernhettes,Herman Höfte +9 more
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TLDR
Mutants at the PROCUSTE1 (PRC1) locus show decreased cell elongation, specifically in roots and dark-grown hypocotyls, suggesting that cellulose synthesis in these organs requires the coordinated expression of at least two distinct cellulose synthase isoforms.Abstract:
Mutants at the PROCUSTE1 (PRC1) locus show decreased cell elongation, specifically in roots and dark-grown hypocotyls. Cell elongation defects are correlated with a cellulose deficiency and the presence of gapped walls. Map-based cloning of PRC1 reveals that it encodes a member (CesA6) of the cellulose synthase catalytic subunit family, of which at least nine other members exist in Arabidopsis. Mutations in another family member, RSW1 (CesA1), cause similar cell wall defects in all cell types, including those in hypocotyls and roots, suggesting that cellulose synthesis in these organs requires the coordinated expression of at least two distinct cellulose synthase isoforms.read more
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Growth of the plant cell wall
TL;DR: Recent discoveries have uncovered how plant cells synthesize wall polysaccharides, assemble them into a strong fibrous network and regulate wall expansion during cell growth.
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Visualization of Cellulose Synthase Demonstrates Functional Association with Microtubules
Alexander R. Paredez,Alexander R. Paredez,Chris Somerville,Chris Somerville,David W. Ehrhardt +4 more
TL;DR: Inhibition of microtubule polymerization changed the fine-scale distribution and pattern of moving CESA complexes in the membrane, indicating a relatively direct mechanism for guidance of cellulose deposition by the cytoskeleton.
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Cellulose Synthesis in Higher Plants
TL;DR: Genetic studies in Arabidopsis have identified a number of genes that contribute to the overall process of cellulose synthesis, but the role of these proteins is not yet known.
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Interactions among three distinct CesA proteins essential for cellulose synthesis
TL;DR: Data suggest that IRX5, IRX3, and IRX1 are all essential components of the cellulose synthesizing complex and the presence of all three subunits is required for the correct assembly of this complex.
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Identification of genes required for cellulose synthesis by regression analysis of public microarray data sets
TL;DR: To identify factors involved in cellulose biosynthesis, a regression method was used to analyze 408 publicly available Affymetrix Arabidopsis microarrays and found the general approach developed here is useful for identification of elements of multicomponent processes.
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