Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp . ATCC 31750
TLDR
A mutant that produced higher levels of curdlan than the wild strain Agrobacterium sp.Abstract:
We isolated a mutant that produced higher levels of curdlan than the wild strain Agrobacterium sp. ATCC 31750 by chemical mutagenesis using N-methyl-N-nitro-nitrosoguanidine. The mutant strain produced 66 g/L of curdlan in 120 h with a yield of (0.88) while, the wild strain produced 41 g/L in 120 h with a yield of (0.62) in a stirred bioreactor. The mutant could not produce curdlan when the pH was shifted from 7.0 to 5.5 after nitrogen depletion as followed for wild strain. In contrast, pH optimum for cell growth and curdlan production for mutant was found to be 7.0. We optimized the downstream processing of curdlan by varying different volumes of NaOH and HCl for extraction and precipitation of curdlan. The molecular weight of the purified curdlan from the wild and mutant strain was 6.6 × 105 Da and 5.8 × 105 Da respectively. The monosaccharide analyses confirm that curdlan from both wild and mutant strain contains only glucose units. From the NMR and FTIR data, it has been confirmed that curdlan was exclusively composed of β (1 → 3)-D-glucan residues.read more
Citations
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A review presenting production, characterization, and applications of biopolymer curdlan in food and pharmaceutical sectors
TL;DR: Curdlan is an exopolysaccharide with a high molecular weight that is made up entirely of monomeric glucose molecules connected by β-1,3-glycosidic bonds.
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Exploiting diol reactivity for the access to unprecedented low molecular weight curdlan sulfate polysaccharides.
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TL;DR: In this article, the authors developed semi-synthetic sequences based on a regioselective protection-sulfation-deprotection approach, allowing the access to some, new, low molecular weight curdlan polysaccharide derivatives with unprecedented sulfation patterns.
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Exopolysaccharide synthesis repressor genes (exoR and exoX) related to curdlan biosynthesis by Agrobacterium sp.
Minjie Gao,Zhi-Lei Liu,Zhongsheng Zhao,Zichao Wang,Xiuyu Hu,Yun-Lin Jiang,Jiajun Yan,Zhitao Li,Zhiyong Zheng,Xiaobei Zhan +9 more
TL;DR: In this article , the roles of exoR and exoX in curdlan biosynthesis in Agrobacterium sp. was explored, and it was shown that ΔexoR was reduced and the motility of this strain increased remarkably compared with the wild type (WT) strain during the cell growth phase.
References
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Journal ArticleDOI
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Book ChapterDOI
Microdetermination of Phosphorus
TL;DR: The method described in this paper has the greatest sensitivity, being able to demonstrate the presence of 0.10 μg of phosphorus per ml. It is based on the reduction of phosphomolybdate complex with ascorbic acid, and is essentially identical to the method published by Chen, Toribara and Warner (1956).
Journal ArticleDOI
Microbial polysaccharides from Gram-negative bacteria
TL;DR: While almost all this non-structural data has accumulated from the study of Gram negative species, this background information provides a sound basis from which polysaccharides from Gram positive bacteria can now be developed.
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