Prospective evaluation of the clinical usefulness of an antigen-specific assay (MAIPA) in idiopathic thrombocytopenic purpura and other immune thrombocytopenias.
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TLDR
The experience suggests that MAIPA assays are useful in the laboratory assessment of thrombocytopenia, should be performed before therapy, and that some patients with 'nonimmune' thromBocy topenia may have genuine antiplatelet antibodies.About:
This article is published in Blood.The article was published on 1996-07-01 and is currently open access. It has received 186 citations till now. The article focuses on the topics: Antigen & Thrombocytopenic purpura.read more
Citations
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Journal ArticleDOI
A modern reassessment of glycoprotein-specific direct platelet autoantibody testing in immune thrombocytopenia
Hanny Al-Samkari,Rachel P. Rosovsky,Rebecca Karp Leaf,David B. Smith,Katayoon Goodarzi,Annemarie E. Fogerty,David B. Sykes,David J. Kuter +7 more
TL;DR: Glycoprotein-specific direct PA testing performed using ISTH recommendations in patients meeting ASH diagnostic criteria is sensitive and specific for ITP diagnosis and reliably confirms clinical remission.
Journal ArticleDOI
CD20+ B-cell depletion therapy suppresses murine CD8+ T-cell–mediated immune thrombocytopenia
Li Guo,Li Guo,Rick Kapur,Rick Kapur,Rukshana Aslam,Edwin R. Speck,Anne Zufferey,Yajing Zhao,Michael Kim,Alan H. Lazarus,Heyu Ni,John W. Semple +11 more
TL;DR: The outcome of B-cell depletion (Bdep) therapy on CD8(+) T-cell-mediated ITP was investigated using a murine model and correlated with normalization of in vivo platelet counts in the transferred SCID mice suggesting that anti-CD20 therapy significantly reduces the ability of CD8 (+) T cells to activate and mediate ITP.
Journal ArticleDOI
Platelet autoantibodies and lupus-associated thrombocytopenia.
Marc Michel,Ketty Lee,Jean-Charles Piette,Patricia Fromont,Annette Schaeffer,Philippe Bierling,Bertrand Godeau +6 more
TL;DR: The production of specific anti‐platelet autoantibodies, mainly directed against GpIIb/IIIa, and their binding to platelet membrane plays an important role in the pathogenesis of SLE‐associated thrombocytopenia.
Journal ArticleDOI
Approach to the Management of Autoimmunity in Primary Immunodeficiency
Gholamreza Azizi,Vahid Ziaee,Marzieh Tavakol,Tina Alinia,Reza Yazdai,Reza Yazdai,Hamed Mohammadi,Hassan Abolhassani,Hassan Abolhassani,Asghar Aghamohammadi +9 more
TL;DR: Management of autoimmunity in patients with PID requires special considerations because dysregulations and dysfunctions of the immune system along with persistent inflammation impair the process of diagnosis and treatment.
Journal ArticleDOI
Drug-induced thrombocytopenia: localization of the binding site of GPIX-specific quinine-dependent antibodies.
TL;DR: The characterization of the quinine-dependent antibody activity of sera from 13 patients with qu inine-induced thrombocytopenia indicated that replacement of the Arg110 and Gln115 of the human GPIX with the corresponding residues from mouse resulted in a significant reduction in the binding of GPIX antibodies in this series of patients.
References
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Monoclonal antibody--specific immobilization of platelet antigens (MAIPA): a new tool for the identification of platelet-reactive antibodies
TL;DR: A new assay is described that takes advantage of monoclonal antibodies against major platelet membrane constituents (glycoproteins IIb/IIIa and Ib and HLA class I molecule) to investigate selectively platelet reactive antibodies against epitopes on different glycoprotein.
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Demonstration of a thrombocytopenic factor in the blood of patients with thrombocytopenic purpura.
TL;DR: Two patients who were demonstrated to have the platelet-reducing factor prior to splenectomy still had the factor present after platelet counts had returned to normal following removal of the spleen, and the thrombocytopenic factor was found in the globulin fraction.
Journal ArticleDOI
Similarities between known antiplatelet antibodies and the factor responsible for thrombocytopenia in idiopathic purpura. physiologic, serologic and isotopic studies
TL;DR: Direct evidence is concerns direct evidence that the ITP factor destroys autologous platelets and is most likely an antibody.
Journal ArticleDOI
Interaction of AP-2, a monoclonal antibody specific for the human platelet glycoprotein IIb-IIIa complex, with intact platelets.
TL;DR: Evidence is obtained that the IIb-IIIa complex exists in the membrane of intact nonstimulated platelets and that complex integrity is not affected by external calcium ion concentration.
Journal ArticleDOI
Platelet-associated and plasma anti-glycoprotein autoantibodies in chronic ITP
TL;DR: It is concluded that clinically adaptable assays allow detection of autoantibodies in most patients with chronic ITP, confirming the presence of an autoimmune process.
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