Journal ArticleDOI
Protein determination of cells immobilized in cross-linked synthetic gels
Amihay Freeman,T. Blank,Y. Aharonowitz +2 more
- Vol. 14, Iss: 1, pp 13-15
TLDR
The procedure worked out was found adequate for the determination of the protein content of microbial cells immobilized in synthetic and native polymer-gel-systems.Abstract:
An assay for the determination of the protein content of whole cells immobilized in cross-linked synthetic gels was developed. The assay is based on a three step procedure: a) methanol dehydration, b) protein extraction by 1.0 M alkali at 125°C c) colorimetric assay of the extracted protein according to Bradford's procedure (Bradford M. M. (1976), Anal. Biochem. 72:248–254). The procedure worked out was found adequate for the determination of the protein content of microbial cells immobilized in synthetic and native polymer-gel-systems.read more
Citations
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Journal ArticleDOI
The immobilization of whole cells: Engineering principles
TL;DR: Techniques which have been used to immobilize whole cells include adsorption, aggregation, confinement and entrapment, and many more have been proposed.
Journal ArticleDOI
Formation of proinsulin by immobilized Bacillus subtilis
TL;DR: It is found that the addition of certain antibiotics in the growth medium, such as novobiocin which inhibits DNA replication, fulfills these requirements, allowing proinsulin synthesis and excretion to take place over a period of several days.
Journal ArticleDOI
Continuous ethanol production by immobilized yeast reactor coupled with membrane pervaporation unit.
Y. Shabtai,S. Chaimovitz,Amihay Freeman,E. Katchalski-Katzir,Charles Linder,Mara Nemas,Mordechay Perry,Ora Kedem +7 more
TL;DR: A system comprised of an immobilized yeast reactor producing ethanol, with a membrane pervaporation module for continuously removing and concentrating the produced ethanol, was developed.
Journal ArticleDOI
The use of confocal scanning laser microscopy and other tools to characterize Escherichia coli in a high-cell-density synthetic biofilm
TL;DR: It is demonstrated that rehydration was an important step in biofilm preparation, and E. coli cast into synthetic biofilms with cell layers of at least 20 to 35 microm in thickness had gene induction characteristics similar to suspended cells.
Journal ArticleDOI
Exopolysaccharide production during batch cultures with free and immobilized Lactobacillus rhamnosus RW-9595M.
TL;DR: Exopolysaccharides (EPS) were produced by Lactobacillus rhamnosus RW‐9595M during pH‐controlled batch cultures with free cells and repeated‐batch cultures with cells immobilized on solid porous supports (ImmobaSil®).
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Nitrogen nutrition and regulation of cephalosporin production in Streptomyces clavuligerus
TL;DR: Antibiotic production was more closely associated with growth in the absence of ammonium than in its presence, indicating a strong inhibitory and (or) repressive effect of NH4+ on antibiotic production.
Journal ArticleDOI
Streptomyces clavuligerus sp. nov., a β-Lactam Antibiotic Producer
C. E. Higgens,R. E. Kastner +1 more
TL;DR: A new species of Streptomyces which produces two new cephalosporin antibiotics is named and described and refers to the production of club-like side branches.
Journal ArticleDOI
Immobilization of microbial cells in crosslinked, prepolymerized, linear polyacrylamide gels: Antibiotic production by immobilized Streptomyces clavuligerus cells
Amihay Freeman,Y. Aharonowitz +1 more
TL;DR: A mild new method for the immobilization whole microbial cells has been developed, which resulted in cells entrapped in gels with physical properties similar to those of the common Polyacrylamide gels.
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Immobilization of microbial cells in crosslinked, prepolymerized, linear polyacrylamide gels: Antibiotic production by immobilized Streptomyces clavuligerus cells
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