Journal ArticleDOI
Protein-flavonol interaction: fluorescence spectroscopic study.
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TLDR
Analysis of relevant spectroscopic data leads to the conclusions that two binding sites are involved in BSA–3HF interaction, and the interaction is slightly positively cooperative in nature with a similar binding constant.Abstract:
Recent studies have shown that various synthetic as well as therapeutically active naturally occurring flavonols possess novel luminescence properties that can potentially serve as highly sensitive monitors of their microenvironments in biologically relevant systems. We report a study on the interactions of bovine serum albumin (BSA) with the model flavonol 3-hydroxyflavone (3HF), using the excited-state proton-transfer (ESPT) luminescence of 3HF as a probe. Upon addition of BSA to the flavonoid solutions, we observe remarkable changes in the absorption, ESPT fluorescence emission and excitation profiles as well as anisotropy (r) values. Complexation of 3HF with protein results in a pronounced shift (20 nm) of the ESPT emission maximum of the probe (from lambda(max)(em) = 513 nm to lambda(max)(em) = 533 nm) accompanied by a significant increase in fluorescence intensity. The spectral data also suggest that, in addition to ESPT, the protein environment induces proton abstraction from 3HF leading to formation of anionic species in the ground state. Fairly high values of anisotropy are observed in the presence of BSA for the tautomer (r = 0.25) as well as anion (r = 0.35) species of 3HF, implying that both the species are located in motion-restricted environments of BSA molecules. Analysis of relevant spectroscopic data leads to the conclusions that two binding sites are involved in BSA-3HF interaction, and the interaction is slightly positively cooperative in nature with a similar binding constant of 1.1 - 1.3 x 10(5) M(-1) for both these sites. Proteins 2001;43:75-81.read more
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Journal ArticleDOI
Interaction of polypyridyl Cr(III) complexes with bovine serum albumin.
Pablo Facundo Garcia,Pablo Facundo Garcia,Consuelo Coronel Arrechea,Consuelo Coronel Arrechea,Alejandra Velo,Alejandra Velo,Julieta Soledad Riva,Julieta Soledad Riva,Lidia Mabel Yudi,Lidia Mabel Yudi,Gerardo A. Argüello,Gerardo A. Argüello +11 more
TL;DR: Experimental results indicate that Cr(NN) 3 3+ binds to serum albumins, by which these proteins could act as carriers of this complex for further applications in PDT.
Journal ArticleDOI
Behind Resveratrol Stabilization by Carboxymethylated (1,3/1,6)-β-d-Glucan: Does the Polyphenol Play a Role in Polymer Structural Organization?
Antonio Francioso,Simone Dinarelli,Marco Girasole,Laura Cervoni,Maria D'Erme,Francesco Mura,Alberto Boffi,Elita Montanari,Luciana Mosca +8 more
TL;DR: Experimental data indicate that CM-glucan conformational organized architecture in aqueous solution is enhanced in the presence of resveratrol, suggesting that the polyphenol is able to confer a high degree of order to the polymer by a probable cooperative structural organization that results in a long term stabilization for thepolyphenol.
Regulation of Trypanosoma brucei hexokinase 1 and 2 on multiple levels: Transcript abundance, protein expression and enzyme activity
TL;DR: This research presents a new approach to evaluate the phytochemical properties of cadmium-3-phosphate and its application in the response to climate change through a simple and scalable approach.
Journal ArticleDOI
Effects of B-ring structures on binding behavior of flavonols with proteins: Experimental and molecular docking approaches
TL;DR: In this article , the effects of the number and position of the hydroxyl groups in flavonols B-ring on the quenching constant, binding constant, number of binding sites, thermodynamic parameters as well as the changes of the secondary structure of proteins are elucidated.
Posted ContentDOI
Facile Green Synthesis of Carbon Dots and Their Utility for Electrochemical Sensing and Binding of a Flavanone, Hesperetin
TL;DR: In this paper , carbon dots modified glassy carbon electrode (C-dots/GCE) were used for analytical and binding applications of a flavanone, hesperetin (HES).
References
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Book
Principles of fluorescence spectroscopy
TL;DR: This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.
Book
Chemistry and biochemistry of plant pigments
TL;DR: This book will help you to understand the chemistry and biochemistry of plant pigments book much better and the system of this book of course will be much easier.
Journal ArticleDOI
Flavonoids, a class of natural products of high pharmacological potency
TL;DR: The few existing reports on the careful pharmacodynamic, pharmacokinetic and clinical studies which have been made have been summarized to provide a basis for a full-scale investigation of the therapeutic potential of flavonoids.
Journal ArticleDOI
Excited state proton-transfer spectroscopy of 3-hydroxyflavone and quercetin
TL;DR: In this article, the double minimum hydrogen-bonding potential of 3-hydroxyflavone and quercetin at room temperature in solution has been used to explain the luminescence of these molecules at 77 K in 2-methylbutane rigid matrix.