Proteoglycans from normal and degenerate cartilage of the adult human tibial plateau
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TLDR
Proteoglycans were extracted from normal and degenerate cartilage of the human tibial plateau and there was no evidence for the changes in glycosaminoglycan synthesis that have been described for clinically osteoarthritic cartilage, or for changes in the ability to aggregate with hyaluronic acid.Abstract:
Proteoglycans were extracted from normal and degenerate cartilage of the human tibial plateau. Both areas possessed proteoglycans of similar chemical composition, though the degenerate cartilage contained a greater proportion of molecules of lower buoyant density and enriched in keratan sulfate. There was no evidence for the changes in glycosaminoglycan synthesis that have been described for clinically osteoarthritic cartilage, or for changes in the ability to aggregate with hyaluronic acid.read more
Citations
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In Vivo and in Vitro Stimulation of Chondrocyte Biosynthetic Activity in Early Experimental Osteoarthritis
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References
More filters
Journal ArticleDOI
A low-viscosity epoxy resin embedding medium for electron microscopy.
TL;DR: A low-viscosity embedding medium based on ERL-4206 is recommended for use in electron microscopy and has a long pot life of several days and infiltrates readily because of its low viscosity.
Journal ArticleDOI
A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
Journal ArticleDOI
The Sialic Acids XI. A PERIODATE-RESORCINOL METHOD FOR THE QUANTITATIVE ESTIMATION OF FREE SIALIC ACIDS AND THEIR GLYCOSIDES
TL;DR: The periodate-resorcinol method was substantially more sensitive than the resorcinl procedure, was not affected by lipids, amino acids, or sugars, and could be used to detect free or glycosidically bound sialic acids on paper chromatograms.
Journal ArticleDOI
The specific interaction of hyaluronic acid with cartilage proteoglycans
Timothy E. Hardingham,Helen Muir +1 more
TL;DR: The addition of small amounts of hyaluronic acid to disaggregated cartilage proteoglyans produced a large increase in hydrodynamic size on gel chromatography and there was no such interaction when proteoglycans were mixed with other polyanions in comparable proportions.
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