Journal ArticleDOI
Proton translocation coupled to formate oxidation in anaerobically grown fermenting Escherichia coli.
TLDR
It is concluded that the hydrogen production by hydrogenase 3 is coupled to formate-dependent proton pumping that regulates 2H(+)-K(+) exchange in fermenting bacteria.About:
This article is published in Biophysical Chemistry.The article was published on 2005-05-01. It has received 54 citations till now. The article focuses on the topics: Formate oxidation & Formate.read more
Citations
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Journal ArticleDOI
A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information.
Adam M. Feist,Christopher S. Henry,Jennifer L. Reed,Markus Krummenacker,Andrew R. Joyce,Peter D. Karp,Linda J. Broadbelt,Vassily Hatzimanikatis,Bernhard O. Palsson +8 more
TL;DR: An updated genome‐scale reconstruction of the metabolic network in Escherichia coli K‐12 MG1655 with increased scope and computational capability is presented, expected to broaden the spectrum of both basic biology and applied systems biology studies of E. coli metabolism.
Journal ArticleDOI
Anaerobic fermentation of glycerol by Escherichia coli: A new platform for metabolic engineering
TL;DR: The finding that Escherichia coli can ferment glycerol in a pH-dependent manner should enable the development of an E. coli-based platform for the anaerobic production of reduced chemicals from Glycerol at yields higher than those obtained from common sugars, such as glucose.
Journal ArticleDOI
Modeling methanogenesis with a genome-scale metabolic reconstruction of Methanosarcina barkeri
TL;DR: This work demonstrates that a reconstructed metabolic network can serve as an analysis platform to predict cellular phenotypes, characterize methanogenic growth, improve the genome annotation and further uncover the metabolic characteristics of methanogenesis.
Journal ArticleDOI
Metabolic pathway engineering for enhanced biohydrogen production
Juanita Mathews,Guangyi Wang +1 more
TL;DR: This review summarizes some of the main biological pathways that produce hydrogen and their limiting factors and describes how metabolic engineering strategies are being used to overcome these limitations, increase yields, and broaden substrate utilization.
Journal ArticleDOI
A new model for the anaerobic fermentation of glycerol in enteric bacteria: trunk and auxiliary pathways in Escherichia coli.
TL;DR: A new model for glycerol fermentation in enteric bacteria is proposed in which the production of 1,2-PDO provides a means to consume reducing equivalents generated in the synthesis of cell mass, thus facilitating redox balance, and the conversion of Glycerol to ethanol, through a redox-balanced pathway, fulfills energy requirements by generating ATP via substrate-level phosphorylation.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Classification and phylogeny of hydrogenases.
TL;DR: Compelling evidence from sequences and structures indicates that the [NiFe]- and [Fe]-H2ases are phylogenetically distinct classes of proteins, which would be consistent with the phylogenetic distinctiveness of the two classes of H2ases.
Journal ArticleDOI
Nucleotide sequence and expression of the selenocysteine-containing polypeptide of formate dehydrogenase (formate-hydrogen-lyase-linked) from Escherichia coli
TL;DR: The results are consistent with a co-translational selenocysteine incorporation mechanism.
Journal ArticleDOI
The respiratory complex I of bacteria, archaea and eukarya and its module common with membrane-bound multisubunit hydrogenases.
Thorsten Friedrich,Dierk Scheide +1 more
TL;DR: The proton‐pumping NADH:ubiquinone oxidoreductase, also called complex I, is the first of the respiratory complexes providing the proton motive force which is essential for energy consuming processes like the synthesis of ATP.
Related Papers (5)
Mutational analysis of the operon (hyc) determining hydrogenase 3 formation in Escherichia coli.
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Energy conservation in chemotrophic anaerobic bacteria.
Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenylase components
R. Böhm,M. Sauter,August Böck +2 more