Purification and amino acid sequence of melanocyte-stimulating hormone from the dogfish Squalus acanthias.
P. J. Lowry,A. Chadwick +1 more
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The sequence of the melanocyte-stimulating hormone, Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Met, shows that ten of its 11 residues are the same as ten of the 13 residues of mammalian alpha-MSH.Abstract:
A melanocyte-stimulating hormone (MSH) was isolated by gel filtration and ion-exchange chromatography from extracts of the pituitary glands of dogfish. Sequence studies were carried out on the hormone and its enzymically and chemically cleaved fragments. The sequence of the hormone, Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Met, shows that ten of its 11 residues are the same as ten of the 13 residues of mammalian α-MSH. About half of its molecules have the carboxyl group at the C-terminus free and about half are amidated; about a fifth have an extra tyrosine residue on the N-terminus, thereby making 11 residues the same as in mammalian α-MSH. Unlike the mammalian hormone, however, none of it was found to be N-acetylated.read more
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Characterization of a Novel α-Amidated Decapeptide Derived from Proopiomelanocortin-A in the Trout Pituitary1
H. Tollemer,H. Tollemer,Jérôme Leprince,Jérôme Leprince,Thierry Bailhache,Thierry Bailhache,Isabelle Chauveau,Isabelle Chauveau,Frans Vandesande,Frans Vandesande,Marie-Christine Tonon,Marie-Christine Tonon,Patrick Jego,Patrick Jego,Hubert Vaudry,Hubert Vaudry +15 more
TL;DR: Two complementary DNAs encoding distinct forms of POMC have been characterized in the trout pituitary, and specific antibodies against the C-terminal pentapeptide Y HFQG and its α-amidated derivative YHFQ-NH2 are developed.
Journal ArticleDOI
Steady-state analysis of α-melanotropin in the pars intermedia of Anolis carolinensis: Effect of background adaptation
TL;DR: The steady-state levels of alpha-melanotropin-stimulating hormone (alpha-MSH)-related peptides were examined in the pars intermedia of the reptile Anolis carolinensis as a function of background adaptation.
Journal ArticleDOI
Chromatographic and electrophoretic characterization of melanocyte-stimulating substances in the duck pituitary.
TL;DR: It is concluded that the bulk of melanotrophic activity of the duck pituitary corresponds to a peptide which behaves as α-MSH in the analytical systems used.
Journal ArticleDOI
Control of pars intermedia activity in late embryos of the spiny dogfish, Squalus acanthias.
Patrick Meurling,B. Klefbohm +1 more
TL;DR: Nearly full-term yolk sac embryos of Squalus acanthias exhibit background adaptation with melanophore responses that are dependent on the pituitary pars intermedia, and an adrenergic release-inhibiting mechanism seems to have a central role.