Journal ArticleDOI
Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor.
Shigetada Nakanishi,Akira Inoue,Toru Kita,Masahiro Nakamura,Annie C. Y. Chang,Stanley N. Cohen,Shosaku Numa +6 more
TLDR
The nucleotide sequence of a 1,091-base pair cloned cDNA insert encoding bovine corticotropin-β-lipotropin precursor mRNA indicates that the precursor protein consists of repetitive units and includes a third melanotropin sequence in its cryptic portion.Abstract:
The nucleotide sequence of a 1,091-base pair cloned cDNA insert encoding bovine corticotropin-beta-lipotropin precursor mRNA is reported. The corresponding amino acid sequence indicates that the precursor protein consists of repetitive units and includes a third melanotropin sequence in its cryptic portion. Pairs of lysine and arginine residues separate the component peptides of the precursor.read more
Citations
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Patterns of Amino Acids near Signal‐Sequence Cleavage Sites
TL;DR: In this paper, some such patterns, based on a sample of 78 eukaryotic signal sequences, are presented and discussed, and a first attempt at formulating rules for the prediction of cleavage sites is made.
Journal ArticleDOI
Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products
TL;DR: A model in which developmental regulation of RNA processing is used to increase the diversity of neuroendocrine gene expression is proposed.
Journal ArticleDOI
Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.
Diane Pennica,Glenn Evan Nedwin,Joel S. Hayflick,Peter H. Seeburg,Rik Derynck,Michael A. Palladino,William J. Kohr,Bharat B. Aggarwal,David V. Goeddel +8 more
TL;DR: Recombinant tumour necrosis factor can be obtained by expression of its complementary DNA in Escherichia coli and induces the haemorrhagic necrosis of transplanted methylcholanthrene-induced sarcomas in syngeneic mice.
Journal ArticleDOI
Galanin — a novel biologically active peptide from porcine intestine
TL;DR: It was found that galanin consists of 29 amino acids and the complete amino acid sequence is: contract smooth muscle preparations from the rat and to cause a mild and sustained hyperglycemia in dog.
Journal ArticleDOI
Endogenous Opioids: Biology and Function
Huda Akil,Stanley J. Watson,Elizabeth A. Young,Michael E. Lewis,Henry Khachaturian,J. M. Walker +5 more
TL;DR: It was therefore impossible for the functionally minded neuro scientist to design and execute experiments taking this heterogeneity into consideration, and even pharmacological studies with these peptides were difficult to interpret in a physiological framework.
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Construction of Biologically Functional Bacterial Plasmids In Vitro
TL;DR: Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genetic properties and nucleotide base sequences from both of the parent DNA molecules.