Purification and amino acid sequence of melanocyte-stimulating hormone from the dogfish Squalus acanthias.
P. J. Lowry,A. Chadwick +1 more
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The sequence of the melanocyte-stimulating hormone, Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Met, shows that ten of its 11 residues are the same as ten of the 13 residues of mammalian alpha-MSH.Abstract:
A melanocyte-stimulating hormone (MSH) was isolated by gel filtration and ion-exchange chromatography from extracts of the pituitary glands of dogfish. Sequence studies were carried out on the hormone and its enzymically and chemically cleaved fragments. The sequence of the hormone, Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Met, shows that ten of its 11 residues are the same as ten of the 13 residues of mammalian α-MSH. About half of its molecules have the carboxyl group at the C-terminus free and about half are amidated; about a fifth have an extra tyrosine residue on the N-terminus, thereby making 11 residues the same as in mammalian α-MSH. Unlike the mammalian hormone, however, none of it was found to be N-acetylated.read more
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α-Melanotropin-like substances in the pituitary and plasma of Xenopus laevis in relation to colour change responses ☆
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Characterization of endorphins from the pituitary of the spiny dogfish Squalus acanthias.
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A view of the N-acetylation of alpha-melanocyte-stimulating hormone and beta-endorphin from a phylogenetic perspective.
TL;DR: A case will be made that there is an “ancestral” POMC N-acetylating mechanism that has generally persisted throughout vertebrate evolution and a novel modification of this “general” scheme that is observed in anuran amphibians will be discussed at length.