Journal ArticleDOI
Purification and characterization of tannin acyl hydrolase from Aspergillus niger MTCC 2425
TLDR
The present investigation was carried out for increasing the yield of tannase of Aspergillus niger and the physico‐chemical characterization of this enzyme, and tannic acid was the best substrate for three substrates tested, followed by methyl gallate and propyl gallate.Abstract:
The present investigation was carried out for increasing the yield of tannase of Aspergillus niger and the physico-chemical characterization of this enzyme. the extraction of enzyme protein. However, extraction of fungal pigments and proteins was observed to have high pH dependence, and maximum enzyme extraction was obtained at pH 5.5. The two-step purification protocol gave 51-fold purified enzyme with a yield of 20%. The total tannase activity was made up of nearly equal activity of esterase and depsidase. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of purified tannase protein indicated it to be made up of two polypeptides of molecular weight 102 and 83 kDa. Based on the Michaelis-Menten constant (Km) of tannase for three substrates tested, tannic acid was the best substrate with Km of 2.8 x 10(-4) M, followed by methyl gallate and propyl gallate. The inhibition was maximum for CaCl2 (58%) whereas EDTA had no modulatory effect on tannase activity. The inhibitor binding constant (KI) of CaCl2 was 5.9 x 10(-4) M Homogenization and detergent pretreatments did not have any remarkable effect on and the inhibition was of noncompetitive type.read more
Citations
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, model integration of the bibliome, genome, metabolome and reactome of Aspergillus niger
TL;DR: This work presents the largest model reconstruction of a metabolic network reported for a fungal species, based on the reportings of 371 articles and comprises 1190 biochemically unique reactions and 871 ORFs, and a graphical map of the metabolic network is presented.
Journal ArticleDOI
Biodegradation of gallotannins and ellagitannins
TL;DR: Biodegradation of tannins is in an incipient stage and further studies have to be carried out to exploit the potential of various tannin for largescale applications in food, fodder, medicine and tannery effluent treatment.
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TL;DR: The investigation of a case of occupational silicosis in Ohio is described and the impact of hospital-based reporting on surveillance forsilicosis is summarized.
Journal ArticleDOI
Ellagic acid production by Aspergillus niger in solid state fermentation of pomegranate residues.
Armando Robledo,Antonio Aguilera-Carbo,Raúl Rodríguez,José L. Martinez,Yolanda Garza,Cristóbal N. Aguilar +5 more
TL;DR: Pomegranate husk is a good support, and at the same time an excellent substrate in the production of high commercial interest metabolites like EA due to the degradation of its ET content.
Journal ArticleDOI
Identification and cloning of a gene encoding tannase (tannin acylhydrolase) from Lactobacillus plantarum ATCC 14917T
TL;DR: Subsequent enzymatic characterization revealed that TanLpl was most active in an alkaline pH range at 40 degrees C, which was quite different from that observed for a fungal tannase of Aspergillus oryzae.
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