Journal ArticleDOI
Purification and composition of the trypsin‐chymotrypsin inhibitors of phaseolus vulgaris l. var rosinha g2
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TLDR
Three trypsin-chymotrypsin inhibitors were purified to homogeneity from Phaseolus vulgaris L. var Rosinha G2 (Brazilian pink beans) and were very similar, although distinguishable, by amino acid analysis.Abstract:
Three trypsin-chymotrypsin inhibitors were purified to homogeneity from Phaseolus vulgaris L. var Rosinha G2 (Brazilian pink beans). At least one more trypsin-chymotrypsin inhibitor was present. Purfication was achieved by fractionation into albumin and globulin fractions based on differential salt/water solubilities, affinity chromatography on trypsin-Sepharose, DEAE-cellulose chromatography and CM-cellulose chromatography. Purity of the inhibitors was determined by rechromatography, combining ratios with trypsin and by disc gel electrophoresis. The isolated inhibitors were very similar, although distinguishable, by amino acid analysis. They had no tryptophan and valine, one residue of methionine, low glycine, alanine, leucine and tyrosine contents and high levels of serine, threonine, proline and half-cystine (18.5 to 21.0 residues per molecular weight of 20,000). They also contained a low amount of carbohydrate (one equivalent of mannose per 20,000 g protein).read more
Citations
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Journal ArticleDOI
The trypsin inhibitors present in seed of different grain legume species and cultivar
Eva Guillamón,Mercedes M. Pedrosa,Carmen Burbano,Carmen Cuadrado,María Sánchez,Mercedes Muzquiz +5 more
TL;DR: Trypsin inhibitors in a selection of grain legume seeds from different species and cultivars were studied and the amount of TI detected, varied with legume species and variety.
Book ChapterDOI
Physical, chemical, and nutritional properties of common bean (Phaseolus) proteins.
TL;DR: The chapter discusses that common beans contain from 18% to 35% protein, however, their biological value is much lower than expected on a protein and amino acid composition basis.
Journal ArticleDOI
Isolation and characterization of trypsin inhibitors from some thai legume seeds
TL;DR: Trypsin inhibitors from cultivars of cowpea, pigeon pea and bambara groundnuts grown in Thailand were isolated and characterized and the extraction time was optimum for the recovery of trypsin inhibitor.
Journal ArticleDOI
HEAT TREATMENT AND INACTIVATION OF TRYPSIN‐CHYMOTRYPSIN INHIBITORS AND LECTINS FROM BEANS (Phaseolus vulgaris L.)
TL;DR: This paper aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about the phytochemical properties of manure and its application in the agricultural sector.
Journal ArticleDOI
Relative importance of phytohemagglutinin (lectin) and trypsin-chymotrypsin inhibitor on bean (Phaseolus vulgaris L) protein absorption and utilization by the rat.
TL;DR: It was assumed that phytohemagglutinin is the main antinutritional and toxic factor that in dry bean (Phaseolus) protein and that trypsin inhibitor (Bowman-Birk type) did not interfere with rat growth.
References
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Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Book ChapterDOI
[117] Chromatographic determination of amino acids by the use of automatic recording equipment
Stanford Moore,William H. Stein +1 more
TL;DR: This chapter describes the chromatographic determination of amino acids by the use of automatic recording equipments and suggests that air-oxidation of cysteine in the hydrolyzate to cystine, before chromatography, is desirable in order to obtain a more accurate value for the total cyStine + cySteine content of the protein.
Journal ArticleDOI
Crystalline soybean trypsin inhibitor
TL;DR: Crystalline soy protein when denatured is readily digestible by pepsin, and less readily by chymotrypsin and by trypsin, which results in a proportional gain in the inhibiting activity.
Journal ArticleDOI
Isolation and properties of a pure yeast polypeptidase
TL;DR: In this article, the enzyme activity of all enzyme preparations was determined by measurement of leucyldiglycine hydrolysis, and the peptidase unit employed, which was identical with the unit used in previous papers, may be defined as the amount of enzyme which will bring about 50 per cent hydrolyisation of I-peptide in 30 minutes.
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