Journal ArticleDOI
Purification and properties of collagen proline hydroxylase from newborn rat skin.
TLDR
Proline hydroxylase and α-ketoglutarate dehydrogenase, which catalyze similar reactions, do not appear to be related enzymologically in that proline hydoxylase does not exhibit a visible spectrum, contains no thiamine pyrophosphate, and does not require any of the cofactors necessary for α.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-08-01. It has received 139 citations till now. The article focuses on the topics: Polyacrylamide gel electrophoresis & Enzyme.read more
Citations
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Journal ArticleDOI
Ascorbic acid: much more than just an antioxidant.
TL;DR: The present paper has tried to bring to the foreground some of the peculiarities of the AA biosynthetic pathway in different organisms, the remarkable function of AA as a co-substrate of many important dioxygenases, the role of AA-regenerating enzymes and the known pathways of AA catabolism.
Book ChapterDOI
Posttranslational enzymes in the biosynthesis of collagen: intracellular enzymes.
Kari I. Kivirikko,Raili Myllylä +1 more
TL;DR: This chapter describes the assay, purification, and properties of the enzymes catalyzing the unique intracellular modifications—i.e., the hydroxylases and hydroxylysyl glycosyltransferases.
Journal ArticleDOI
The biosynthesis of collagen. 1.
TL;DR: Micro-Heterogeneity in the Biosynthesis of Collagen Detailed analyses of the structure of the polypeptide chains of collagen has revealed a "micro-heterogeneity" of the amino acid sequences.
Journal ArticleDOI
Hepatic fibrosis. Correlation of biochemical and morphologic investigations
H Popper,S Uenfriend +1 more
TL;DR: Hepatic fibrosis probably stimulated by hepatocellular injury may, in turn, damage the hepatic parenchyma, and this may be more important in the self-perpetuation of liver injury than the immunologic components of hepatic inflammation.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
Journal ArticleDOI
A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures
Robert G. Martin,Bruce N. Ames +1 more
TL;DR: Sucrose gradient centrifugation is found to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures and the sedimentation behavior of several of the enzymes in the pathway of histidine biosynthesis in S. typhimurium has been determined.