Journal ArticleDOI
Purification and properties of follicle-stimulating and luteinizing hormones from horse pituitary glands.
W.E. Braselton,W.H. McShan +1 more
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A method is described for the purification of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from horse pituitary glands and a number of LH components and an FSH component with a different electrophoretic mobility than the major horse FSH fraction were seen.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-07-01. It has received 88 citations till now. The article focuses on the topics: Isoelectric focusing & Sephadex.read more
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Isoelectric points of proteins: A table
TL;DR: A computerized bibliography from the MEDLARS data base covering the time period of January 1970-March 1976 was retrieved and a table of isoelectric points (pZ) of proteins selected from the literature was prepared.
Patent
Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
Gary Durack,Jeffrey D. Wallace,Gary P. Vandre,Lon A. Westfall,Jeremy T. Hatcher,Niraj V. Nayak +5 more
TL;DR: In this article, a multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of radiation and a beam splitter for producing multiple beams of radiation for each interrogation location associated with each flow channel of the system.
Journal ArticleDOI
Preparation of gonadotropin from salmon (Oncorhynchus tshawytscha) pituitary glands.
TL;DR: A procedure is described for the purification of pituitary gonadotropin from the salmon by means of ethanol extraction, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE-cellulose; (preparations SG-DEAE-1 to 7).
Journal ArticleDOI
Influence of Photoperiod and Ovaries on Seasonal Reproductive Activity in Mares
TL;DR: There was a seasonal pattern of LH and FSH in ovariectomized mares and the LH levels increased approximately 2 months earlier in the light-treated group than in the control group, and the rise in LH in the OVX groups occurred at approximately the same time as the onset of the ovulatory season in the intact groups.
Journal ArticleDOI
Purification of equine gonadotropins and comparative study of their acid-dissociation and receptor-binding specificity.
Florian Guillou,Yves Combarnous +1 more
TL;DR: The method for the simultaneous purification of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from equine pituitaries is briefly described and the data obtained are consistent with the ‘negative specificity’ model proposed recently.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Principles and Procedures of Statistics.
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Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
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Determination of serum proteins by means of the biuret reaction.
TL;DR: An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.