Journal ArticleDOI
Purification and some properties of L-ascorbic-acid-specific peroxidase in Euglena gracilis Z.
TLDR
The results indicate that the Euglena peroxidase belongs to a new type of peroxIDase to be designated as l -ascorbic acid per oxidase, and the inhibition of the enzyme by cyanide and azide as well as absorption spectra of the enzymes alone and in complex with hydrogen peroxide showed that it is a hemoprotein.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1980-04-15. It has received 78 citations till now. The article focuses on the topics: Ascorbic acid & Cytochrome c peroxidase.read more
Citations
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Journal ArticleDOI
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Yoshiyuki Nakano,Kozi Asada +1 more
TL;DR: Observations confirm that the electron donor for the scavenging of hydrogen peroxide in chloroplasts is L-ascorbate and that the L-ASCorbate is regenerated from DHA by the system: photosystem I-*ferredoxin-*NADP^>glutathione and a preliminary characterization of the chloroplast peroxidase is given.
Journal ArticleDOI
Ascorbate peroxidase – a hydrogen peroxide-scavenging enzyme in plants
TL;DR: Amino acid sequence and other molecular properties indicate that ascorbate peroxidase resembles cytochrome c peroxIDase from fungi rather than guaiacol per oxidase from plants, and it is proposed that the plant and yeast hydrogen peroxide-scavenging peroxidsases have the same ancestor.
Journal ArticleDOI
Regulation and function of ascorbate peroxidase isoenzymes
Shigeru Shigeoka,Takahiro Ishikawa,Masahiro Tamoi,Yoshiko Miyagawa,Toru Takeda,Yukinori Yabuta,Kazuya Yoshimura +6 more
TL;DR: It is clear that a high level of endogenous ascorbate is essential effectively to maintain the antioxidant system that protects plants from oxidative damage due to biotic and abiotic stresses.
Journal ArticleDOI
Glutathione metabolism and possible biological roles in higher plants
TL;DR: Synthesis of glutathione in plants seems to proceed in the same series of enzyme catalysed reactions observed in animal cells; the pathway ofglutathione may be the same as that of animal cells.
Book ChapterDOI
Chloroplasts: formation of active oxygen and its scavenging
TL;DR: Production of O2- and H2O2 and their scavenging system are discussed in this chapter and are believed to be indispensable for the prevention of overreduction of electron carriers in the cyclic electron transport pathway.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
TL;DR: The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.
Journal ArticleDOI
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations
TL;DR: A nomenclature to facilitate discussion of possible mechanisms for enzyme-catalyzed reactions with more than one substrate or product is proposed and examples are given to illustrate the types of information about reaction mechanisms that can be obtained.
Related Papers (5)
Ascorbate Peroxidase in Tea Leaves: Occurrence of Two Isozymes and the Differences in Their Enzymatic and Molecular Properties
Gong-Xiang Chen,Kozi Asada +1 more
Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical
Yoshiyuki Nakano,Kozi Asada +1 more