Journal ArticleDOI
Purification of wheat proteases by affinity chromatography on hemoglobin-Sepharose column.
G.K. Chua,W. Bushuk +1 more
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TLDR
A two-fold increase in specific activity was achieved by this purification technique, and all of the non-proteolytic components were separated from the proteolytically active proteins.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1969-10-22. It has received 66 citations till now. The article focuses on the topics: Sepharose & Affinity chromatography.read more
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Journal ArticleDOI
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.
TL;DR: It is demonstrated that successful application of affinity chromatography in many cases will critically depend on placing the ligand at a considerable distance from the matrix backbone.
Journal ArticleDOI
Nutritional improvement of cereals by sprouting
J K Chavan,S S Kadam +1 more
TL;DR: The magnitude of the nutritional improvement is influenced by the type of cereal, seed quality, sprouting conditions, and it is not large enough to account for in feeding experiments with higher animals.
Journal ArticleDOI
The Purification of β-Galactosidase from Escherichia coli by Affinity Chromatography
TL;DR: The ability of the enzymatically inactive monomer forms to bind to the ligand column suggests that this affinity chromatographic method may be used in the purification of catalytically inactive mutant forms which retain the ability to bind substrate analogues.
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The use of selective adsorbents in capillary electrophoresis-mass spectrometry for analyte preconcentration and microreactions: A powerful three-dimensional tool for multiple chemical and biological applications
TL;DR: The concept of biological recognition and affinity interaction on‐line with high‐resolution separation, the fabrication of an “analyte concentrator‐microreactor”, optimization conditions of adsorption and desorption, the coupling to mass spectrometry, and various applications of clinical and pharmaceutical interest are described.
Journal ArticleDOI
Agarose derivatives for purification of protein by affinity chromatography.
TL;DR: Beaded agarose (‘Sepharose’) is a useful insoluble support which has recently been used successfully in the selective purification of enzymes1–5,7,9, antibodies and antigens10–15, chemically synthesized peptides16,17, thyroxine binding serum proteins and in the study of the interaction of insulin with cell membranes.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.