Journal ArticleDOI
Radiation-induced autophosphorylation of epidermal growth factor receptor in human malignant mammary and squamous epithelial cells.
Reads0
Chats0
TLDR
The data presented identify the epidermal growth factor receptor protein tyrosine kinase associated with the plasma membrane as one target for ionizing radiation in the dose range used in radiotherapy.Abstract:
In an effort to identify events initiating up-regulation of epidermal growth factor receptor after single and repeated radiation exposures, we investigated the role of epidermal growth factor receptor, a receptor protein tyrosine kinase, in radiation-induced signal transduction. Human malignant mammary, MCF-7, and squamous, A431, cells showed low baseline phospho-tyrosine levels of epidermal growth factor receptor, permitting reproducible dose-dependent stimulation of epidermal growth factor receptor autophosphorylation after exposure to epidermal growth factor. MCF-7 cells exhibited a mean 2.3-fold increase (95% confidence interval: 1.91, 2.65; P < 0.0001) in levels of epidermal growth factor phosphorylation in response to exposures of 2 Gy, which was substantially less than the epidermal growth factor receptor Y phosphorylation induced by epidermal growth factor. A quantitatively similar radiation response was seen in A431 cells. In the dose range of 1 to 4 Gy, no clear dose response was seen. There was a rapid induction of radiation-induced epidermal growth factor receptor Y phosphorylation, starting within 2 min, with maximum values between 0.5 and 5 min after radiation exposure followed by a slower decline to baseline levels after 20 min. The data presented identify the epidermal growth factor receptor protein tyrosine kinase associated with the plasma membrane as one target for ionizing radiation in the dose range used in radiotherapy.read more
Citations
More filters
Journal Article
Irradiated Mammary Gland Stroma Promotes the Expression of Tumorigenic Potential by Unirradiated Epithelial Cells
TL;DR: Data indicate that radiation-induced changes in the stromal microenvironment can contribute to neoplastic progression in vivo, and disruption of solid tissue interactions is a heretofore unrecognized activity of ionizing radiation as a carcinogen.
Journal ArticleDOI
Stress and radiation-induced activation of multiple intracellular signaling pathways.
Paul Dent,Adly Yacoub,Joseph N. Contessa,Rubén W. Carón,George Amorino,Kristoffer Valerie,Michael P. Hagan,Steven Grant,Rupert Schmidt-Ullrich +8 more
TL;DR: This review will attempt to describe some of the complex network of signals induced by ionizing radiation and other cellular stresses in animal cells, with particular attention to signaling by growth factor and death receptors.
Journal ArticleDOI
Epidermal growth factor receptor in glioma: signal transduction, neuropathology, imaging, and radioresistance.
TL;DR: Recent progress in key aspects of EGFR signaling in GBM are discussed, focusing on neuropathology, signal transduction, imaging of the EGFR, and the role of theEGFR in mediating resistance to radiation therapy in G BM.
Journal Article
Inverse Relationship between Epidermal Growth Factor Receptor Expression and Radiocurability of Murine Carcinomas
TL;DR: The pretreatment assessment of EGFR expression could predict radiotherapy outcome and may assist in selecting an effective treatment modality.
Journal ArticleDOI
Signal transduction and cellular radiation responses
TL;DR: There is increasing evidence for the functional links between cellular signal transduction responses and DNA damage recognition and repair, cell survival, or cell death through apoptosis or reproductive mechanisms.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Journal ArticleDOI
Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells
Tony Hunter,Jonathan A. Cooper +1 more
TL;DR: Increased phosphorylation of tyrosine is the most rapid response of cells to EGF known, and may play an important role in the biological effects of EGF.
Journal ArticleDOI
Ionizing radiation regulates expression of the c-jun protooncogene.
TL;DR: The present studies demonstrate transcriptional activation of a mammalian gene, the c-jun protooncogene, by x-rays and suggest a role for induction of early response genes in the pathophysiologic effects of ionizing radiation.
Journal ArticleDOI
Protein kinase C mediates x-ray inducibility of nuclear signal transducers EGR1 and JUN
Dennis E. Hallahan,Vikas P. Sukhatme,Matthew L. Sherman,Subbulakshmi Virudachalam,Donald Kufe,Ralph R. Weichselbaum +5 more
TL;DR: Analysis of mRNA expression for different classes of immediate early genes after cellular x-irradiation implicate the EGR1 and JUN proteins as signal transducers during the cellular response to radiation injury and suggest that this effect is mediated in part by a protein kinase C-dependent pathway.