Radioimmunoassay of gastrin
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TLDR
A highly specific radioimmunoassay for endogenous human plasma gastrin was developed using guinea pig antisera to crude porcine gastrin using highly purified 125 I-porcine Gastrin I as tracer and an anionic exchange resin was used to separate bound and free labeled gastrin.About:
This article is published in Gastroenterology.The article was published on 1970-01-01 and is currently open access. It has received 727 citations till now. The article focuses on the topics: Big gastrin & Gastrin.read more
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Journal ArticleDOI
Gastric Acid Secretion Rate and Buffer Content of the Stomach after Eating. RESULTS IN NORMAL SUBJECTS AND IN PATIENTS WITH DUODENAL ULCER
John S. Fordtran,John H. Walsh +1 more
TL;DR: It was shown that the rate of acid secretion in response to a steak meal in seven duodenal ulcer patients was twice the rate achieved in six control subjects and that the amount of acid secreted after eating exceeded the peak histamine response in the ulcers but not in the controls.
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Production and evaluation of antibodies for the radioimmunoassay of gastrin.
TL;DR: Only three antisera had antibodies with sufficient binding energy for radioimmunochemical determination of low physiologic concentrations of serum-gastrin (K0≥1011l/mol), and one of them reacted with cholecystokinin to an extent that made it unsuited for gastrin determinations in biologic fluids.
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Structural and hormonal alterations in the gastrointestinal tract of parenterally fed rats.
Leonard R. Johnson,Edward M. Copeland,Stanley J. Dudrick,Lenard M. Lichtenberger,Gilbert A. Castro +4 more
TL;DR: Results suggest that maintenance of normal tissue stores of the hormone, gastrin, is dependent on stimuli provided by oral ingestion and the presence of food in the gastrointestinal tract and the disproportionate decrease in weight that occurs in certain tissues is apparently unrelated to the absence of nutrients.
Journal ArticleDOI
Zollinger-Ellison syndrome.
References
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Journal ArticleDOI
The preparation of 131i-labelled human growth hormone of high specific radioactivity
TL;DR: The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.
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Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
TL;DR: Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.
Journal ArticleDOI
Immunoassay of Endogenous Plasma Insulin in Man
TL;DR: For years investigators have sought an assay for insulin which would combine virtually absolute specificity with a high degree of sensitivity, sufficiently exquisite for measurement of the minute insulin concentrations usually present in the circulation as mentioned in this paper.
Journal ArticleDOI
Antibodies to bradykinin and angiotensin: a use of carbodiimides in immunology.
TL;DR: Antibodies to bradykinin and angiotensin have been produced in rabbits by the use of conjugates containing albumin and the hapten, covalently bound using water-soluble carbodiimide reagents.
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The constitution and properties of two gastrins extracted from hog antral mucosa.
R. A. Gregory,Hilda J. Tracy +1 more
TL;DR: This paper is the culmination of many years' work on the detection, isolation, physiological study, and now, large-scale production of gastrin, and the final product has been identified as two almost identical peptides (gastrins I and II).
Related Papers (5)
The constitution and properties of two gastrins extracted from hog antral mucosa.
R. A. Gregory,Hilda J. Tracy +1 more
Gastric Acid Secretion Rate and Buffer Content of the Stomach after Eating. RESULTS IN NORMAL SUBJECTS AND IN PATIENTS WITH DUODENAL ULCER
John S. Fordtran,John H. Walsh +1 more