Journal ArticleDOI
Radioprotective effect of 2-mercaptopropionylglycine on radiation-induced microsomal lipid peroxidation.
S.I. Ayene,P.N. Srivastava +1 more
Reads0
Chats0
TLDR
The exogenous supply of Fe2+ during irradiation was found to eliminate the 'threshold dose' which has to be delivered before MPG shows an 'enhancement effect'.Abstract:
SummaryRadiation-induced microsomal lipid peroxidation was measured following γ-irradiation (66–399 Gy) in the presence or absence of 2-mercaptopropionylglycine (MPG) at low and high concentrations (0·03 and 0·1 mg/ml). At high concentrations of MPG, enhancement of lipid peroxidation was observed instead of reduction. Following γ-irradiation at 266·4 Gy, lipid peroxidation increased with increasing concentrations of MPG. The spontaneous lipid peroxidation also increased with increasing concentrations of MPG. When MPG and EDTA were added together, radioprotection was observed even at high concentrations of MPG after different doses of radiation. An increase in radioprotection with increasing concentrations of MPG in the presence of EDTA at 266·4 Gy was also observed. The exogenous supply of Fe2+ during irradiation was found to eliminate the ‘threshold dose’ which has to be delivered before MPG shows an ‘enhancement effect’. A possible mechanism for the enhancement of radiation-induced lipid peroxidation is...read more
Citations
More filters
Journal ArticleDOI
Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes.
R Varshney,R K Kale +1 more
TL;DR: Rat liver microsomes were irradiated with gamma-rays at a dose rate of 1.31 Gys-1.31 and calmodulin antagonists considerably inhibited radiation-induced lipid peroxidation in the presence of ferric (Fe3+) ions, which decreased the cytochrome P-450 content of microsome.
Journal Article
Radiation induced peroxidative damage: mechanism and significance.
Anjali Agrawal,Raosaheb K. Kale +1 more
TL;DR: The basic mechanism of radiation induced lipid peroxidation is addressed and efforts are made to show similarities between radiolytic and non-radiolytic lipidperoxidation.
Journal ArticleDOI
Radioprotective effect of 2-mercaptopropionyl glycine on radiation-induced lipid peroxidation and enzyme release in erythrocytes.
TL;DR: GAMma-Irradiation of erythrocyte suspensions resulted in lipid peroxidation and enzyme (acetylcholinesterase, AChE) release and the presence of 2-mercaptopropionyl glycine during irradiation indicated that lipid per oxidation may induce the breakdown of the phosphatidylinositol/enzyme interaction.
Journal ArticleDOI
Amino acids and their derivatives as radioprotective agents.
TL;DR: The study of amino acids and their derivatives as radioprotective agents continues to contribute to an understanding of processes involved in radiation toxicity and to offer new compounds with potential application to situations of human exposure.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Formation of a thiobarbituric-acid-reactive substance from deoxyribose in the presence of iron salts
TL;DR: The role of this radical @ iron-dependent damage to the sugar deoxyribose is examined, showing that treatment of several amino acids or carbohydrates with iron (II) salts in phosphate buffer causes formation of a product that reacts with thiobarbituric acid (TBA) to produce a chromogen.
Journal ArticleDOI
Superoxide-dependent formation of hydroxyl radicals in the presence of iron chelates: is it a mechanism for hydroxyl radical production in biochemical systems?
Journal ArticleDOI
Inhibition of the iron-catalysed formation of hydroxyl radicals from superoxide and of lipid peroxidation by desferrioxamine
TL;DR: The iron chelator desferrioxamine inhibits peroxidation at all concentrations tested, and it also inhibits the iron-catalysed formation of hydroxyl radicals from superoxide (O2-.).
Related Papers (5)
Radiation-induced Lipid Peroxidation and Membrane-bound Enzymes in Liver Microsomes
Osami Yukawa,Tohru Nakazawa +1 more