Raman spectroscopy in head and neck cancers: toward oncological applications.
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TLDR
Findings of this study indicate that with the instrument in vivo Raman spectra in finger print region can be recorded in short and clinically implementable time.Abstract:
Aims: To test the spectral reproducibility of earlier findings under ex vivo conditions and to record good quality in vivo spectra in clinically implementable time in finger print region. Materials and Methods: Spectra from 20 ex vivo tissues (10 normal and 10 tumor) were recorded using fiber optic probe coupled Raman spectrometer. In vivo spectra from 10 healthy volunteers were also recorded. Spectral differences were analyzed by PC-LDA method followed by validation by leave-one-out and test samples. Results: Spectral features of ex vivo normal tissue suggest predominant lipid features while protein is high in tumor conditions. Major spectral features of in vivo healthy volunteers share several commonalities with ex vivo normal tissues except minor differences in amide III region. Classification efficiency of 90, 85 and 96% for ex vivo tumor, normal and in vivo normal standard models, respectively after leave-one-out cross validation, was observed. Test prediction efficiency of standard models of ex vivo normal, ex vivo tumor and in vivo healthy volunteers were 82.5, 92.5 and 100%, respectively. Conclusions: Our findings corroborate with the reported ex vivo and in vivo normal spectral features. Features of in vivo Raman spectra show strong similarities with ex vivo normal spectra minor but significant differences were also observed. Findings of this study indicate that with our instrument in vivo Raman spectra in finger print region can be recorded in short and clinically implementable time.read more
Citations
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In vivo Raman spectroscopic identification of premalignant lesions in oral buccal mucosa.
TL;DR: Results suggest that premalignant conditions can be objectively discriminated with both normal and cancerous sites as well as from healthy controls with and without tobacco habits, and further support efficacy of Raman spectroscopic approaches in oral-cancer applications.
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Label-free characterization of exosome via surface enhanced Raman spectroscopy for the early detection of pancreatic cancer.
Joseph Carmicheal,Chihiro Hayashi,Xi Huang,Lei Liu,Yao Lu,Alexey V. Krasnoslobodtsev,Alexander Y. Lushnikov,Prakash Kshirsagar,Asish Patel,Maneesh Jain,Yuri L. Lyubchenko,Yongfeng Lu,Surinder K. Batra,Sukwinder Kaur +13 more
TL;DR: Utility of SERS spectral signature for deciphering exosomal surface signature is identified for detection of pancreatic cancer.
Journal ArticleDOI
Raman spectroscopy in head and neck cancer
Andrew T. Harris,Andrew Rennie,Haroon Waqar-Uddin,Sarah R Wheatley,Samit K Ghosh,Dominic P Martin-Hirsch,Sheila E. Fisher,Sheila E. Fisher,Alec S. High,Jennifer Kirkham,Tahwinder Upile,Tahwinder Upile +11 more
TL;DR: Raman spectroscopy could confer great patient benefit with early, rapid and accurate diagnosis, and could reduce the need for whole pathological specimen examination, in theatre it could help to determine margin status, and finally peripheral blood diagnosis may be an achievable target.
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Investigation of the potential of Raman spectroscopy for oral cancer detection in surgical margins
Froukje L.J. Cals,Tom C. Bakker Schut,Jose A. Hardillo,Robert J. Baatenburg de Jong,Senada Koljenović,Gerwin J. Puppels +5 more
TL;DR: This study shows how well Raman spectroscopy enables discrimination between OCSCC and surrounding healthy tissue structures, and supports the development of robust and reliable classification algorithms for future implementation of RamanSpectroscopy in clinical practice.
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Raman micro-spectroscopy for rapid screening of oral squamous cell carcinoma.
Luis Felipe das Chagas e Silva de Carvalho,Franck Bonnier,Kate O'Callaghan,Jeff O'Sullivan,Stephen Flint,Hugh J. Byrne,Fiona M. Lyng +6 more
TL;DR: This technique may provide a rapid screening method and have potential use in the diagnosis of dysplasia and early, non-invasive oral cancer, the treatment of which involves much less extensive and complex surgery and a reduction in associated co-morbidity for the patient.
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Non-Invasive Raman Spectroscopic Detection of Carotenoids in Human Skin
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TL;DR: The first evidence that carotenoid concentration in the skin correlate with the presence or absence of skin cancer and precancerous lesions is presented, using a noninvasive optical method, Raman spectroscopy.