Journal ArticleDOI
Rapid detection of mecA and nuc genes in staphylococci by real-time multiplex polymerase chain reaction.
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TLDR
This rapid mecA/nuc RT-PCR assay is a suitable and practical tool for the routine detection of methicillin resistance and identification of S. aureus, which can be easily incorporated into the diagnostic molecular microbiology laboratory work flow.About:
This article is published in Diagnostic Microbiology and Infectious Disease.The article was published on 2005-01-01. It has received 109 citations till now. The article focuses on the topics: Multiplex & Multiplex polymerase chain reaction.read more
Citations
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Journal ArticleDOI
First case of Staphylococcus pseudintermedius infection in a human.
TL;DR: The first clinical report of a Staphylococcus pseudintermedius infection in a human is presented and the final microbiological identification requires the combination of phenotypic and genotypic tests.
Journal ArticleDOI
Rapid methods for diagnosis of bloodstream infections.
Mariam Klouche,Uwe Schröder +1 more
TL;DR: In this article, a review summarises the currently available data on rapid non-culture-based technologies and outlines the potential clinical usefulness in infectious disease diagnosis, including the potential to reduce morbidity, mortality, and of the economic burden.
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Genotyping of methicillin-resistant Staphylococcus aureus (MRSA) isolated from milk and dairy products in South Italy
TL;DR: The detection of MRSA in food of animal origin is a potential health hazard, thus it is necessary monitoring of food-producing animals and improving hygiene standards in food practices in order to reduce the microbiological risk to minimum.
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Evaluation of the IDI-MRSA Assay for Detection of Methicillin-Resistant Staphylococcus aureus from Nasal and Rectal Specimens Pooled in a Selective Broth
Marc Desjardins,Marc Desjardins,Christiane Guibord,B. Lalonde,Baldwin Toye,Baldwin Toye,Karam Ramotar,Karam Ramotar +7 more
TL;DR: The IDI-MRSA assay performed well for the detection of MRSA from a selective broth compared to the performance of the detection from culture, however, because of the burden associated with implementation of infection control precautions, cultures remain essential in confirming positive IDi- MRSA results.
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Bench-to-bedside review: Rapid molecular diagnostics for bloodstream infection - a new frontier?
TL;DR: This review summarises the currently available technologies, their strengths and limitations and the obstacles that have to be overcome in order to develop a satisfactory bedside point-of-care diagnostic tool for detection of bloodstream infection.
References
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Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks.
TL;DR: Elimination of carriage of S. aureus has been found to reduce the infection rates in surgical patients and those on hemodialysis and CAPD, and those colonized with MRSA.
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Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification of Staphylococcus aureus
TL;DR: A DNA-based test provides a novel diagnostic tool for the diagnosis of S. aureus infections and is simple and can be performed in about 1 h, while the PCR assay was specific and ubiquitous for the identification from bacterial cultures of 195 clinical strains of the bacterium.
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Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR.
P. Vannuffel,J. Gigi,H. Ezzedine,Bernard Vandercam,Michel Delmée,Georges Wauters,Jean-Luc Gala +6 more
TL;DR: Coamplification of mecA and femA determinants proved to be very reliable both for rapid detection of methicillin resistance and differential diagnosis between S. aureus and other staphylococci.
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Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay
Patrice Francois,Didier Pittet,Manuela Bento,Beatrice Pepey,Pierre Vaudaux,Daniel Pablo Lew,Jacques Schrenzel +6 more
TL;DR: A rapid procedure was developed for detection and identification of methicillin-resistant Staphylococcus aureus directly from sterile sites or mixed flora samples, allowing prompt and cost-effective implementation of contact precautions.
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Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR.
TL;DR: A duplex LightCycler PCR assay targeting the mecA gene and a Staphylococcus a Aureus-specific marker was used to test 165 S. aureus strains and 80 strains of other bacterial species.