Journal ArticleDOI
Rat hippocampal neurons in dispersed cell culture
G A Banker,W. M. Cowan +1 more
TLDR
There is a clear relationship between the stage of the cells' development and their growth in culture and in the presence of hippocampal explants the initial growth of the isolated cells is improved and their survival in culture is extended to about two weeks.About:
This article is published in Brain Research.The article was published on 1977-05-13. It has received 1135 citations till now. The article focuses on the topics: Trypsinization & Cell aggregation.read more
Citations
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Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination
TL;DR: High survival was achieved with osmolarity lower than found in Dulbecco's Modified Eagle's Medium (DMEM), and by reducing cysteine and glutamine concentrations and by the elimination of toxic ferrnous sulphate found in DME/F12, and Neurobasal is a new medium that incorporates modifications to DMEM.
Journal ArticleDOI
Neurotrophic and neurotoxic effects of amyloid beta protein: reversal by tachykinin neuropeptides
TL;DR: The amyloid beta protein could function as a neurotrophic factor for differentiating neurons, but at high concentrations in mature neurons, as in Alzheimer's disease, could cause neuronal degeneration.
Journal ArticleDOI
The establishment of polarity by hippocampal neurons in culture
TL;DR: By the end of the first week in culture, hippocampal neurons have established a single axon and several dendrites, which is a stereotyped sequence of developmental events that lead to the establishment of this characteristic form.
Journal ArticleDOI
Neurodegeneration induced by beta-amyloid peptides in vitro: the role of peptide assembly state
TL;DR: Aggregation properties of an overlapping series of synthetic beta-amyloid peptides were investigated and compared with beta AP neurotoxic properties in vitro, finding that few beta APs assembled into aggregates immediately after solubilization, but that over time peptides containing the highly hydrophobic beta 29–35 region formed stable aggregations.
Journal ArticleDOI
Culturing hippocampal neurons
Stefanie Kaech,Gary Banker +1 more
TL;DR: This protocol provides protocols for preparing low-density dissociated-cell cultures of hippocampal neurons from embryonic rats or mice that become appropriately polarized, develop extensive axonal and dendritic arbors and form numerous, functional synaptic connections with one another.
References
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Journal ArticleDOI
The autoradiographic demonstration of axonal connections in the central nervous system.
W.M. Cowan,W.M. Cowan,D.I. Gottlieb,D.I. Gottlieb,Anita E. Hendrickson,Anita E. Hendrickson,J.L. Price,J.L. Price,T.A. Woolsey,T.A. Woolsey +9 more
TL;DR: There are several reasons for thinking that this method may offer a number of advantages over other currently available techniques, and it should be possible to do this by locally injecting radioactively labeled precursors of proteins or other macromolecules into the brain or spinal cord.
Journal ArticleDOI
An autoradiographic study of the time of origin and the pattern of granule cell migration in the dentate gyrus of the rat
TL;DR: Examination of the final location of granule cells labeled at different stages reveals three distinct morphogenetic gradients in the gyrus, which suggest that cells in the dorsal blade tend to be formed earlier than those in the ventral blade; cells inThe more caudal portions of the g Cyrus are generated earlier thanThose in more rostral (or septal) regions; and in all regions the more superficial neurons in the stratum granulosum are form earlier than the
Journal ArticleDOI
Attachment and culture of dissociated cells from rat embryo cerebral hemispheres on polylysine-coated surface.
Ephraim Yavin,Ziva Yavin +1 more
TL;DR: This report presents a method for circumventing selection problem by allowing the majority of cells to adhere by the use of a polylysine pretreated surface, and it is based upon previous observations on the electrostatic interaction of the polycation with the negative charges of the cell membrane.
Journal ArticleDOI
Possible roles for cell-to-substratum adhesion in neuronal morphogenesis.
TL;DR: The correlation of strong adhesion and enhanced neuronal morphogenesis suggests that adhesive interactions between the growth cone and the microenvironment in an embryo are crucial parts of the initiation and elongation of neuronal processes.
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The establishment of polarity by hippocampal neurons in culture
Growth of a rat neuroblastoma cell line in serum-free supplemented medium.
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